参芪扶正注射液对脐血CIK细胞体外增殖及杀瘤活性的影响

目的观察参芪扶正注射液对脐血细胞因子诱导的杀伤细胞(CIK细胞)体外增殖及杀瘤活性的影响。方法用脐血单个核细胞,诱导分化CIK细胞,分为4组。细胞因子组加入γ干扰素(IFN-γ)、白细胞介素1α(IL-1α)、白细胞介素2(IL-2)、CD3单抗细胞因子;参芪扶正注射液组:加入参芪扶正注射液;细胞因子+参芪扶正注射液组:加入参芪扶正注射液﹑IFN-γ、IL-1α、IL-2、CD3单抗细胞因子;对照组只加培养液。培养12天后CIK细胞达到增殖高峰期,用流式细胞仪检测细胞表型和增殖能力,MTT法检测CIK细胞对K562细胞的杀伤作用,ELISA法检测CIK细胞分泌的细胞因子IL-2、IFN-γ、肿瘤坏死因子α(TNF-α)水平。结果对脐血CIK细胞体外增殖的影响,在培养12天时,参芪扶正注射液组与细胞因子组比较差异无统计学意义(P>0.05),细胞因子+参芪扶正注射液组与细胞因子组相比差异有统计学意义(P<0.01);CIK细胞对K562细胞的杀伤作用,各实验组与对照组相比差异有统计学意义(P<0.01),参芪扶正注射液组CIK细胞在12、16天时分泌IL-2、IFN-γ、TNF-α因子水平优于对照组(P<0.01)。结论参芪扶正注射液对脐血CIK细胞既有显著的增殖和杀瘤作用,又可促进脐血CIK细胞分泌IFN-γ、IL-2和TNF-α。

Effect of Shenqi Fuzheng Injection on Proliferation and Cytotoxity of CIK Cells Originated from Umbilical Cord Blood

Objective To investigate the effect of Shenqi Fuzheng Injection(Ginseng and Astragalus Right-Supporting Injection) on proliferation and cytotoxity of cytokine-induced killer(CIK) cells originated from umbilical cord blood.Methods CIK cells were induced and differentiated from the cord blood mononuclear cells and then were divided into Cytokine group,Shenqi Fuzheng Injection group,Cytokine plus Shenqi Fuzheng Injection group and the control group.Cytokine group was added γ-interferon(IFN-γ),interleukin(IL)-1α,IL-2 and CD3.Shenqi Fuzheng Injection group was added Shenqi Fuzheng Injection.Cytokine plus Shenqi Fuzheng Injection group was added Shenqi Fuzheng Injection and IFN-γ,IL-1α,IL-2 and CD3.The control group was added only cluture solution.After 12 days of cultivation,the proliferation of CIK cells reached the peak.The phenotype and proliferative capacity of CIK cells was detected with flow cytometry,the cytotoxity was detected by MTT assay,and the level of IL-2,IFN-γ and tumor necrosis factor(TNF)-α was detected by ELISA assay.Results On the 12th day of cultivation,there was no significant difference in the proliferation of CIK cells between Cytokine group and Shenqi Fuzheng Injection group(P>0.05),but with significant difference between Cytokine group and Cytokine plus Shenqi Fuzheng Injection group(P<0.01).The killing effect of CIK cells against K562 cells in Cytokine group,Shenqi Fuzheng Injection group and Cytokine plus Shenqi Fuzheng Injection group was better than that of the control group(P<0.01).CIK cells in Shenqi Fuzheng Injection group could secrete IL-2,IFN-γ and TNF-α.Conclusion Shenqi Fuzheng Injection could improve the proliferation and cytotoxicity of CIK cells and promote the secretion of IFN-γ,IL-2 and TNF-α as well.