硫氧还蛋白对缺氧/复氧大鼠海马神经元caspase-3活性的影响

目的：探讨硫氧还蛋白（Trx)和caspase-3在鼠海马神经元缺氧／复氧损伤中的作用。方法：原代培养10d的新生鼠海马神经元缺氧3h复氧12h,24h和48h，分为正常对照组、缺氧／复氧组和Trx组，用MTT比色法测定各时点的神经元存活率；底物裂解法检测caspase-3活性。结果：缺氧／复氧组海马神经元复氧12－48h，海马神经元caspase-3样蛋白酶活性明显增高，以复氧24h为最高，而存活率进行性下降；Trx组caspase-3样蛋白酶活性曲线与缺氧／复氧组相似，但明显下降，细胞存活率较缺氧／复氧组明显提高。结论：海马神经元缺氧／复氧后，caspase-3样蛋白酶被激活，参与神经元损伤过程；Trx对神经元有保护作用。

The Effect of Thioredoxin on Caspase-3 in Rat Hippocampal Neurons by Hypoxia/Reoxygenation

Objective To study the roles of thiorodoxin and caspase-3 in rat hippocampus neurons following hypoxia/reoxygenation. Methods Ten-day-old neuronal cells were divided into three groups, the rat hippocampal neurons were induced hypoxia for 3 hours, followed by 12 to 48 hours of reoxygenation in two groups, and one being pretreated with thioredoxin for 1 hour; the left group was control group. The neuronal viability was estimated by MTT assay, and caspase-3 activities were measured by a colorimetric method. Results The caspase-3 was activated in rat hippocampal neurons during hypoxia/reoxygenation, and caspase-3 like activity was significantly increased after reoxygenation, with the peak at 24 hours of reoxygenation. Decreased caspase-3 like activity was detected in the neurons pretreated with thioredoxin. On the other hand, the neuronal viability was decreased in H/R group, but was improved in thioredoxin group. Conclusion caspase-3 contributes to the neuronal damage following hypoxia/reoxygenation; and thioredoxin has the neuroprotective effects.