Identification, isolation, and characterization of a species-specific 30-kDa antigen of Oesophagostomum dentatum |
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| Authors: | A. Joachim B. Ruttkowski C. M. Christensen A. Daugschies |
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| Affiliation: | (1) Institute of Parasitology, Hannover School of Veterinary Medicine, Bünteweg 17, D-30559 Hannover, Germany E-mail: ardaug@parasit.tiho-hannover.de, Tel.: +49-511-953 8794, Fax: +49-511-953 8870, DE;(2) Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Ridebanevej 3, DK-1870 Frederiksberg C, Denmark, DK |
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| Abstract: | In the search for a serology tool for the diagnosis of nonpatent as well as patent infections with Oesophagostomum dentatum in pigs a water-soluble, unglycosilated antigen of about 30 kDa specific for the third-stage larvae of the parasite was purified by ion-exchange chromatography. In Western blots, the antigen was first detected by antibodies at day 7 postinfection. Cross-reactivity with O. quadrispinulatum, Ascaris suum, or Trichuris suis was not detected. It is suggested that this protein is a suitable tool for the species-specific serodiagnosis of O. dentatum infection in pigs. Received: 15 June 1998 / Accepted: 28 September 1998 |
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| Keywords: | Abbreviations L3 Third-stage larvae L4 Fourth-stage larvae SDS Sodium dodecyl sulfate PAGE polyacrylamide gel electrophoresis IEP Isoelectric point IEF Isoelectric focusing FPLC Fast protein liquid chromatography |
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