The Hansenula polymorpha per6 mutant is affected in two adjacent genes which encode dihydroxyacetone kinase and a novel protein, Pak1p, involved in peroxisome integrity |
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| Authors: | I. J. van der Klei Meis van der Heide Richard J. S. Baerends Karl-Björn Rechinger Klaas Nicolay J. A. K. W. Kiel Marten Veenhuis |
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| Affiliation: | (1) Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, NL-9751 NN Haren, The Netherlands Tel.: +31-50-3632179 Fax: +31-50-3635205 e-mail: IJVDKLEI@BIOL.RUG.NL, NL;(2) University of Utrecht, Padualaan 8, NL-3584 CH Utrecht, The Netherlands, NL;(3) Carlsberg Laboratory, Department of Physiology, Gamle Carlsberg Vej 10, DK-2500 Valby, Copenhagen, Denmark, DK |
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| Abstract: | ![]()
The Hansenula polymorpha per6-210 mutant is impaired in respect of growth on methanol (Mut–) and is characterized by aberrant peroxisome formation. The functionally complementing DNA fragment contains two open reading frames. The first encodes dihydroxyacetone kinase (DAK), a cytosolic enzyme essential for formaldehyde assimilation; the second ORF codes for a novel protein (Pak1p). We have demonstrated that per6-210 cells lack DAK activity, causing the Mut– phenotype, and have strongly reduced levels of Pak1p, resulting in peroxisomal defects. Sequence analysis revealed that per6-210 contains a mutation in the 3′ end of the DAK coding region, which overlaps with the promoter region of PAK1. Possibly this mutation also negatively affects PAK1 expression. Received: 25 February / 12 May 1998 |
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| Keywords: | Peroxisome biogenesis Methanol metabolism Yeast |
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