Identification of a common conserved neutralizing linear B-cell epitope in the VP3 protein of waterfowl parvoviruses |
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| Authors: | Caiyu Lian Ruihua Zhang Jingjing Lan Yupeng Yang Hanqing Li Nana Sui |
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| Affiliation: | 1. Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian, People’s Republic of China;2. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, People’s Republic of China;3. Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Taian, People’s Republic of China;4. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, People’s Republic of China |
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| Abstract: | ABSTRACT Waterfowl parvoviruses (WPVs) including goose parvovirus (GPV), novel GPV-related virus (NGPV) and Muscovy duck parvovirus (MDPV) cause significant economic losses and epizootic threat to the waterfowl industries, and little is known about the B-cell epitopes of WPVs. In this study, a monoclonal antibody (mAb) 5B5 against the VP3 protein of NGPV was used to identify the possible epitope in the three kinds of WPVs. The mAb 5B5 had neutralizing activities to the three viruses, and reacted with the conserved linear B-cell epitopes of 438LHNPPP443 in VP3 protein of GPV, NGPV and MDPV. To the authors’ best knowledge, this is the first report on identification of the common conserved neutralizing linear B-cell epitope on VP3 protein of three different WPVs, which would facilitate the development of a novel immunodiagnostic assay for rapid detection of WPV infection. |
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| Keywords: | Goose parvovirus novel goose parvovirus-related virus Muscovy duck parvovirus monoclonal antibody VP3 protein epitope |
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