葡多酚对大鼠肝脏TNF-α与TGF-β_1mRNA异常表达的抑制作用

目的: 研究葡多酚(GPC)对N-亚硝基化合物引发的大鼠肝细胞肿瘤坏死因子α(TNF-α)mRNA与转化生长因子β1(TGF-β1)mRNA异常表达的抑制作用。 方法:将大鼠随机分为正常对照组、阳性对照组和2个GPC实验组,每组10只,雌雄各半。后3组给口服50 mg/kg bw亚硝酸钠诱发突变,2个GPC实验组经口分别给予GPC100 mg/kg bw和10 mg/kg bw,喂养8 w,体内灌注固定肝组织,用原位杂交技术检测肝细胞TNF-αmRNA与TGF-β1 mRNA的表达水平。 结果: 阳性对照组TNF-αmRNA与TGF-β1 mRNA表达的阳性细胞率分别为30.23%和19.47 %,高剂量GPC组则为11.94 %和6.96 %,低剂量GPC组分别为18.16%和14.03%,与阳性对照组之间的差别均有显著性意义(P<0.05)。结论: 葡多酚对N-亚硝基化合物引发的大鼠肝细胞TNF-αmRNA与TGF-β1 mRNA的异常表达均有抑制作用。

EFFECT OF GRAPE PROCYANIDIN ON N-NITRO COMPOUNDS-INDUCED EXPRESSION OF TNF-α mRNA AND TGF-β1 mRNA IN THE HEPATIC CELLS OF RATS

Objective: To study the effect of grape procyanidin (GPC) on N-nitroso compounds-induced expression of TNF- mRNA and TGF-β1 mRNA in hepatic cells. Methods: NaNO2 was infused into Wistar rats by 50 mg/kg bw to induce mutation. GPC was infused by 100mg/kg bw and 10mg/kg bw respectively through mouth into the rats of two experimental groups. After 8 w situ hybridization was applied to measure the expression level of TNF-?mRNA and TGF-β1 mRNA in hepatic cells. Results: The expression rate of TNF-醡RNA and TGF-β1 mRNA of the hepatic cells in positive control group was 30.23 % and 19.47 % respectively, while that of high-dose GPC group was 11.94 % and 6.96 % respectively .The differences were statistically significant (P<0.05). Conclusion: GPC has inhibitory effect on N-nitroso compounds-induced abnormal expression of both TNF-?mRNA and TGF-β1 mRNA in hepatic cells.