| 异体脂肪源间充质干细胞移植后稳定嵌合体的形成及免疫耐受的诱导 |
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| 引用本文： | 顾冰,马保根,房佰俊,宋永平.异体脂肪源间充质干细胞移植后稳定嵌合体的形成及免疫耐受的诱导[J].河南职工医学院学报,2008,20(5):423-426. |
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| 作者姓名： | 顾冰 马保根 房佰俊 宋永平 |
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| 作者单位： | 郑州大学第一附属医院研究生处,河南,郑州,450052 |
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| 摘 要： | 目的研究器官移植中脂肪源F1k1＋CD31—CD34-细胞诱导免疫耐受的可能性。方法以CM—DiI荧光染料示踪脂肪源F1k1＋CD31-CD34-细胞的体内分布情况，并以PCR方法检测Y染色体的存在以进-步鉴定；以皮肤移植实验观察脂肪源F1k1＋CD31-CD34-细胞移植组小鼠对供者来源组织器官移植物的反应性。结果脂肪源F1k1＋CD31-CD34-细胞可进入并较长期（30d）存在于异基因小鼠免疫器官内；在细胞移植组小鼠脾脏中可检测到供者来源T细胞，占受者脾细胞6．68％；脂肪源F1k1＋CD31-CD34-细胞移植组小鼠供者来源皮肤移植物存活〉90d，未处理组平均为10d。结论异基因脂肪源F1k1＋CD31-CD34-细胞移植后可形成稳定嵌和体，并诱导特异性免疫耐受的产生。
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| 关 键 词： | 干细胞 移植 免疫耐受 嵌合体 脂肪 |
Allogeneic Adipose Tissue-derived Mesenchymal Stem Cells Transplantation Leads to Stable Chimerism and Induces Donor-specific Tolerance |
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| GU Bing,MA Bao-gen,FANG Bai-jun,SONG Yong-ping.Allogeneic Adipose Tissue-derived Mesenchymal Stem Cells Transplantation Leads to Stable Chimerism and Induces Donor-specific Tolerance[J].Journal of Henan Medical College For Staff and Workers,2008,20(5):423-426. |
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| Authors: | GU Bing MA Bao-gen FANG Bai-jun SONG Yong-ping |
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| Institution: | GU Bing,MA Bao-gen FANG Bai-jun,SONG Yong-ping (Department of Hematology,The First Affiliated Hospital of Zhengzhou University,Zhengzhou Henan 450052,China) |
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| Abstract: | Objective To investigate the possibility of immune tolerance induced by adipose tissue-derived Flk1+CD31-CD34-mesenchymal stem cells(MSC).Methods In this research,the writers used an available fluorescence dye,CM-DiI,as a tracing label,and Y-chromosome-specific PCR to detect the distribution of adipose tissue-derived Flk1+CD31-CD34-MSC cells in vivo.Skin transplantation test was performed to observe immune response of Flk1+CD31-CD34-MSC cells-transplanted group mice against organ graft from donor mice.Resul... |
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| Keywords: | Stem cell transplantation immune tolerance chimerism adipose |
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