159对接受助孕治疗不孕症夫妇支原体和衣原体感染情况分析

目的探讨接受助孕治疗的不孕症夫妇支原体和衣原体携带情况及其与体外受精／卵细胞质内单精子注射结局的关系。方法选择2009年3月至2011年9月北京大学第一医院生殖与遗传医疗中心无下生殖道感染症状且接受助孕治疗的159对不孕症夫妇，在取卵日或人工授精日分别取宫颈分泌物和精液，采用聚合酶链反应（PCR）法进行支原体和衣原体的检测。结果女性患者支原体阳性29例（18．200，29／159），其中解脲支原体（ureaplasma urealyticum，UU）和人型支原体（mycoplasma hominis，Mh）分别为26例（16．4％，26／159）和3例（1．9％，3／159），未检出衣原体。男性患者支原体阳性4例（2．5％，4／159），均为UU；衣原体阳性3例（1．9％，3／159）。输卵管性不孕症患者支原体阳性10例（16．9％，10／59），非输卵管性不孕症患者支原体阳性19例（19．0％，19／100），两者比较，差异无统计学意义（P〉0．05）。女性支原体阴性组和阳性组的获卵数（11．8±6．0）个，（14．6±6．2）个]、优质胚胎率（37．6％，43．2％）和临床妊娠率（48．2％，30．0％）比较，差异无统计学意义（P〉0．05）。结论进入助孕治疗周期的不孕症夫妇仍有一定的生殖道支原体和衣原体携带率，支原体携带与临床妊娠结局无明显关系。

Detection of mycoplasma and chlamydia trachomatis in 159 infertility couples undergoing assisted reproductive technology

Objective To determine the prevalence of mycoplasma and chlamydia trachomatis （CT） in endo cervical swab and semen samples collected from women and men undergoing ART, and to explore the relationship between the mycoplasma and IVF/ICSI outcome in women. Methods A total of 159 cervical swab and 159 semen samples collected were investigated. The mycoplasma and CT were examined by polymerase chain reaction （PCR）. Results Among 159 cervical swab samples, mycoplasma were detected in 29 （18.2%, 29/159）. The frequency of ureaplasma urealyticum （UU） and mycoplasma hominis （Mh） were 16.4% （26/159） and 1.9% （3/159）, respec tively. There was no CT. Among the semen samples, mycoplasma and CT were detected in 4 （2.5%, 4/159）, 3 （1.9%, 3/159）, respectively. There was no relationship between pathogens and the TFI. There was no significant difference in retrieved follicles （11.8±6.0, 14.6±6.2）, high- quailty embyro rate （37.6% vs 43.2%） and pregnancy rate （48.2% vs 30.0%） between mycoplasma negative group and positive group. Conclusion There was still a certain carriage ratioof mycoplasma and CT in fertility couples who were ready to accept ART. There was no obvious relationship between mycoplasma carriage ratio with clinic pregnancy rate.