北沙参对免疫抑制C57BL/6J小鼠T淋巴细胞亚群影响的实验研究

目的研究北沙参超临界二氧化碳(SFE-CO2)萃取物对免疫抑制C57BL/6J小鼠T淋巴细胞亚群的影响。方法将实验用C57BL/6J雌性小鼠32只,随机分为空白组、模型组、北沙参SFE-CO2萃取物低、高剂量组,每组8只。第7天处死小鼠,眼静脉取血,EDTA抗凝,血球计数仪自动检测WBC、RBC、PLT;流式细胞术检测,计算T细胞亚群绝对数量。结果北沙参SFE-CO2萃取物可显著提高免疫抑制C57BL/6J小鼠外周血中WBC、PLT、CD3+T、CD4+T和CD8+T细胞的绝对数量。结论北沙参SFE-CO2萃取物对免疫抑制C57BL/6J小鼠外周免疫系统有较显著的恢复作用。

The study on T lymphocyte subpopulation influence of Glehnia littoralis Schmidt on the C57BL/6J immunosuppressive mice

Objective: To study the T lymphocyte subpopulation influence of Glehnia littoralis Schmidt SFE-CO2 extract on the C57BL/6J immunosuppressive mice.Methods: Thirty-two normal C57BL/6J female mice were divided randomly into the blank group,model group,low-Glehnia littoralis Schmidt SFE-CO2 extract-dose group and high-Glehnia littoralis Schmidt SFE-CO2 extract-dose group,(each n=8).All the mice were killed and the orbit venous sampling was done at the seventh day.EDTA was used as an anticoagulant.WBC,RBC,PLT were tested by globulimeter automatically.The absolute quantity of T lymphocyte subpopulation was tested and calculated by flow cytometry.Results: The Glehnia littoralis Schmidt SFE-CO2 extract obviously improved the absolute quantity of WBC,PLT,CD3+T,CD4+T and CD8+T cells in the peripheral blood of C57BL/6J immunosuppressive mice.Conclusion: The Glehnia littoralis Schmidt SFE-CO2 extract has special rehabilitation for the peripheral immune system of the C57BL/6J immunosuppressive mice.