Immunohistochemical detection of human lung and gastric cancer antigen in human salivary gland tumors

Immunohistochemical identification of human lung and gastric carcinoma antigen detected with monoclonal antibodies (MoAbs) KM-93 and KM-231 respectively, was described in 83 salivary gland tumors, including 67 pleomorphic adenomas, 5 adenolymphomas, 3 mucoepidermoid tumors, 6 sialadenocarcinomas, and 2 adenoid cystic carcinomas as well as in normal salivary glands. The binding patterns of these two MoAbs was compared with that of MoAb recognizing epithelial membrane antigen (EMA). Serous cells of normal salivary glands showed positive KM-93 staining, whereas ductal cells were positive with KM-231, with ductal basal cells being characteristically so. EMA staining was confined to luminal and lateral borders of serous acini and ducts. Pleomorphic adenomas indicated positive depositions for both KM-93 and KM-231 in luminal tumor cells or luminal borders of tubuloductal structures. Adenolymphomas showed positive KM-231 staining in basal tumor cells and a positive KM-93 reaction in luminal tumor cells. Mucoepidermoid tumors revealed positive KM-231 staining in mucous-secreting cells, whereas weak KM-93 staining was found in all tumor cells. Sialoadenocarcinomas exhibited varying degrees of positive staining with KM-93 and KM-231 in their neoplastic cells. Adenoid cystic carcinomas showed luminal staining with KM-93 and KM-231 to their neoplastic cells. Adenoid cystic carcinomas showed luminal staining with KM-93 and MoAb EMA. The histogenesis of these salivary gland tumors is discussed in terms of the immunohistochemical features of staining patterns obtained with MoAbs KM-93 and KM-231.