“二袖套法”大鼠原位肝移植的技术改进

目的 探讨大鼠原位肝移植(OLT)模型的技术改进方法,并观察移植后的排斥反应。方法 将“二袖套法”大鼠肝移植技术进行了改进;并行SD→SD,SD→Wistar大鼠肝移植各30例,观察术后排斥情况。结果 全组肝移植手术无肝期约为15min。大鼠无手术死亡。SD-SD大鼠肝移植后3周内存活率为97％;SD→Wistar大鼠肝移植后8～15d死亡,组织病理学证实存在不同程度的排斥反应。结论 采用该改良大鼠肝移植方法可明显缩短手术时间,降低术后并发症,提高移植大鼠的术后生存率。SD-Wistar大鼠的肝移植可作为较理想的研究肝移植排斥反应的动物模型。

Technical improvement of "two-cuff method" of orthotopic liver transplantation in rats

ObjectiveTo investigate an improved technique of orthotopic liver transplantation with the ("two-cuff method") in rats, and to observe allograft rejection after transplantation. Methods30 Wistar rats were grafted with livers of SD rats and 30 SD rats were grafted with livers of SD rats by using a modified "two-cuff technique" and examined for postoperative rejection. ResultsThe anhepatic period was about 15 minutes. The 24-hour survival rate was 100%. The Wistar rats grafted with SD livers died 8 to 15 days (after) liver transplantation, and the histopathologic examination showed various degrees of allograft rejection. The SD rats grafted with livers of SD rats had a survival rate of 97% at 3 weeks postoperatively. ConclusionsThe modified "two-cuff technique" can effectively reduce operative time, decrease postoperative (complications) and increase the survival rate of othortopic liver transplantation in rats. The transplantation model of Wistar rats grafted with livers of SD rats can be used to study allograft rejection.