Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer |
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| Authors: | Michaela Frye Ilaria Dragoni Suet-Feung Chin Immaculada Spiteri Agata Kurowski Elena Provenzano Andrew Green Ian O. Ellis Donna Grimmer Andrew Teschendorff Christos C. Zouboulis Carlos Caldas Fiona M. Watt |
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| Affiliation: | 1. Wellcome Trust Centre for Stem Cell Research, Tennis Court Road, Cambridge CB2 1QR, UK;2. CR-UK Cancer Research Technology Ltd., Sardinia House, Sardinia Street, London WC2A 3NL, UK;3. CR-UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK;4. Cambridge Breast Unit and NIHR Cambridge Biomedical Research Centre, Cambridge CB2 2SP, UK;5. Division of Pathology, School of Molecular Medical Sciences, University of Nottingham and Nottingham University Hospitals NHS Trust, Nottingham NG5 1PB, UK;6. Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, 06847 Dessau, Germany |
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| Abstract: | We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target. |
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| Keywords: | RNA methyltransferase Myc Gene amplification Breast cancer |
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