菟丝子中槲皮素含量的高效液相色谱法测定

目的优化并建立以高效液相色谱法测定菟丝子中槲皮素含量的方法。方法采用超声法从菟丝子中提取得到槲皮素,经薄层层析法、紫外分光光度法等方法进行定性分析并采用高效液相色谱法(HPLC)测定槲皮素的含量。色谱柱为YWG C18(250 mm×4.6 mm,10μm),流动相为甲醇,检测波长256 nm,柱温为30℃,流速为1 ml.min-1。结果用该方法测定槲皮素线性范围为8～24μg.ml-1。相关系数r=0.999 6,平均加样回收率为98.96%。RSD为2.47%(n=9)。结论该方法简便、准确、可靠,可作为菟丝子中槲皮素的含量测定方法。为菟丝子的进一步开发利用和有效性研究提供理论依据。

Determination of Quercetin in Cuscuta chinensis by HPLC

Objective To optimize and develop a determination method for content of Quercetin in Cuscuta chinensis by HPLC. Methods The extraction of Quercetin was performed using ultrasonic extraction technology,the production was identified by TLC and UV-spectrophotometry and was determined by HPLC.The YWG C18(250 mm×4.6 mm,10 μm)column was adopted with methanol as the mobile phase,the flow rate was 1.0 ml·min-1,the detection wavelength was set at 256nm,and the column temperature was 30℃. Results The linear range of Quercetin was within the range of 8~24 μg·ml-1l(r=0.999 6) and the recovery rate was 98.96%,RSD was 2.4%(n=9). Conclusion The method is simple and rapid to determine the content of quercetin in Cuscuta chinensis and it provides scientific basis for the safety and effectiveness of Cuscuta chinensis in its clinical use.