玉郎伞多糖提取工艺的正交法优化

目的对玉郎伞提取工艺进行改进,利用正交法进一步优化提取玉郎伞多糖提取工艺。方法采用正交法优化玉郎伞的提取工艺;以苯酚-硫酸显色法于490 nm波长处测定葡萄糖含量吸光度,计算换算因子和玉郎伞多糖含量。结果葡萄糖的浓度在15～65μg/ml范围内其吸光度呈良好的线性关系(r=0.999 1);平均加样回收率为98.25%,RSD=1.72%(n=6);平均换算因子f=1.82,RSD=2.16%(n=6),玉郎伞多糖的平均提取率为3.78%,RSD=0.173 8%(n=9)。多糖的最佳提取条件为浸泡3 h、加10倍量水、提取3次每次3 h。结论优化提取方法后,玉郎伞多糖的提取率提高98%。苯酚-硫酸法可作为玉郎伞多糖定量分析。

Optimization of Extractioin Process of Polysaccharides from Yulangsan

Objective To optimize the extraction process of polysaccharides from Yulangsan by orthogonal optimization method. Methods Optimum extraction process for polysaccharides was investigated by orthogonal design.Phenol-sulfuric acid method was used,and the absorbance was measured at the wavelength of 490nm. Results Phenol-sulfuric acid method showed good precision and linearity,the average recovery ratio was 98.25%,and RSD=1.72%(n=6).The optimal conditions were as follows:three hours of water-soaking time,adding ten time amount of water,three hours of extration time and extracting three times. Conclusion Under the optimun conditions,the yield of polysaccharides from Yulangsan can be enhanced by 98%.Phenol-sulfuric acid method can be used for determination of polysaccharides from Yulangsan.