ＪＷＡ基因-７６Ｇ＞Ｃ和４５４Ｃ＞Ａ多态性与宫颈癌易感性

目的:探讨JWA基因多态性与江苏人群宫颈癌易感性之间的关系.方法:采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)方法,检测227例宫颈癌患者和333例年龄、性别相匹配的非肿瘤者JWA-76G＞C、454C＞A和723T＞G多态性的频率和分布.比较不同基因型携带者患宫颈癌的危险性,通过分层分析探讨初潮年龄、初次生育年龄和产次对患宫颈癌的影响.结果:JWA-76GC和454AA基因型均可增加罹患宫颈癌的危险性.携带3-5个危险等位基因者比携带0-2个等位基因者患官颈癌的危险性更大(OR=1.70.95%CI=1.20～2.42).分层分析结果显示,初潮年龄早、生育年龄晚和产次多且携带3-5危险等位基因者能增加患宫颈癌的危险性.本研究未发现JWA基因723T＞G多态性与宫颈癌之间存在显著相关.结论:JWA基因-76G＞C和454C＞A多态性显著增加江苏地区汉族人群患宫颈癌的危险性.

JWA gene -76G>C and 454C>A polymorphisms significantly increase the risk of cervical cancer in Jiangsu population

Objective:To investigate the association between of JWA gene single nucleotide polymorphisms and the risk of cervical cancer. Methods:In a hospital-based case-control study of 227 cervical patients and 333 cancer-free controls frequency-matched by age,the genotype of the -76G>C,454C>A and 723T>G polymorphisms of JWA gene were detected by the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method;the interaction of menarche age,reproductive age for the first time(RAFT) and parity were further analyzed. Results:A significant increased risk of cervical cancer was found to be associated with the -76GC genotype compared with the -76GG genotype(OR=1.84,95% CI=1.21~2.78),and 454AA genotype compared with the 454CC genotype(OR=1.84,95% CI=1.08~3.14). Moreover,individuals with 3~5 risk alleles will increase the risk of cervical cancer accompanied with early menarche age,late RAFT and many parity. There was no association found between JWA 723T>G polymorphism and cervical cancer. Conclusion:The JWA-76G>C and 454C>A polymorphisms might positively contribute to the etiology of cervical cancer.