| 鲍曼不动杆菌及其耐药基因的检测 |
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| 引用本文： | 张小洁 肖春红 袁建芬 等. 鲍曼不动杆菌及其耐药基因的检测[J]. 中国医药科学, 2014, 0(2): 25-28 |
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| 作者姓名： | 张小洁 肖春红 袁建芬 等 |
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| 作者单位： | [1] 江苏省如皋市人民医院,江苏如皋,226500 [2] 江苏省南通市肿瘤医院,江苏南通,226000 [3] 江苏省南通市中医院,江苏南通,226001 |
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| 摘 要： | 目的：探索一种检测鲍曼不动杆菌及其耐药基因的新方法。方法首先对近几年医院内鲍曼不动杆菌的耐药情况进行分析，选择临床上治疗鲍曼不动杆菌的首选药，再采用聚合酶链反应对鲍曼不动杆菌及其突变株进行检测。结果鲍曼不动杆菌对亚胺培南的耐药率最低,为9.37%，OXA-51基因扩增结果与细菌培养相符，OXA-23基因扩增与鲍曼不动杆菌耐亚胺培南的结果相符。结论采用PCR法对OXA-51基因扩增可以检测是否存在鲍曼不动杆菌，采用PCR法对OXA-23基因扩增可以检测鲍曼不动杆菌是否存在亚胺培南耐药性。从而快速检测鲍曼不动杆菌及其耐药基因，指导临床用药。
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| 关 键 词： | 细菌 耐药性 聚合酶链反应 鲍曼不动杆菌 |
Acinetobacter baumannii and its drug resistance gene detection |
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| ZHANG Xiaojie,XIAO Chunhong,YUAN Jianfen,HONG Hong,YU Haizhong. Acinetobacter baumannii and its drug resistance gene detection[J]. China Medicine and Pharmacy, 2014, 0(2): 25-28 |
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| Authors: | ZHANG Xiaojie XIAO Chunhong YUAN Jianfen HONG Hong YU Haizhong |
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| Affiliation: | 1.Rugao People＇s Hospital,Rugao 226500,China;2.Tumor Hospital of Nantong City,Nantong 226000,China; 3.Traditional Chinese Medicine Hospital of Nantong City, Nantong 226001,China) |
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| Abstract: | Objective To develop the method of detecting Acinetobacter baumannii （A.baumannii ） and its drug-resistance gene. Methods PCR was used to detect the bacteria and its drug-resistance gene for the epidemiological study of A. baumannii infection in the hospital. Results The resistance rate of A.baumannii against imipenem was 9.37%,the lowest one in all antibiotics being used for treating A. baumannii infection.The gene of OXA-51 and OXA-23 were consistent with the bacteria identification and drug resistance respectively. Conclusion PCR for OXA-51 and OXA-23 gene can be used to detect the A. baumannii and its imipenem resistance gene .And it can applied in the rapid diagnosis and clinical treatment guidance. |
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| Keywords: | Bacteria Drug resistance Polymerase chain reaction Acinetobacter baumannii |
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