精氨酸增强全肠外营养对急性胰腺炎大鼠肠粘膜屏障的影响

目的：探讨精氨酸增强精氨酸增强ＴＰＮ对急性胰腺炎（ＡＰ）大鼠肠粘膜屏障的影响。方法：雄性ＳＤ大鼠６４只,随机分成：①对照组（ｎ＝１６）;②ＡＰ组（ｎ＝１６）;③ＡＰ＋ＴＰＮ组（ＴＰＮｓ组ｎ＝１６）;④ＡＰ＋ＴＰＮ＋精氨酸组（ＴＰＮａ组ｎ＝１６）。分别于建立急性胰腺炎模型后第１及第５天剖杀每组８只大鼠取材,检测肠粘膜一氧化氮（ＮＯ）、丙二醛、蛋白质含量、肠系膜淋巴结及门静脉血细菌移位率。结果：与对照组比较,术后１天及５天时,ＡＰ组肠粘膜丙二醛焦点量及肠系膜淋巴结细菌移位率显著升高;５天时蛋白质含量显著降低,而门静脉血细菌移位率明显升高。与ＴＰＮｓ组比较,ＴＰＮａ组１天时肠粘膜ＮＯ含量即明显升高;肠系膜淋巴结细胞移位率显著降低;５天时肠粘膜ＮＯ与蛋白质含量均显著升高;丙二醛含量显著降低。结论：急性胰腺炎可引起肠粘膜

Effect of arginine enriched TPN on gut barrier of rats with acute pancreatitis

Objectives:To investigate the effect of arginine enriched TPN on gut barrier of rats with acute pancreatitis. Methods:64 SD rats were divided into four groups at random:1.Control group(sham operated, n =16);2.AP group( n =16);3.AP+TPN group(TPNs group, n =16);4.AP+TPN+Arginine group (TPNa group, n =16).Every 8 rats in each group were sacrificed on day 1 and day 5 after inducing acute pancreatitis respectively.The level of nitric oxide(NO),malondialdehyde,protein in intestinal mucosa and bacterial translocation rates were measured. Results:Compared with control group on day 1 and day 5 after induction of acute pancreatitis.The level of malondialdehyde in intestinal mucosa and bacterial translocation rates of mesenteric lymphaden of AP group increased significantly( P <0.01, P <0.05);The level of protein in intestinal mucosa decreased and bacterial translocation rates in portal vein increased significantly on day 5( P <0.01).Compared with TPNs group,the level of NO in intestinal mucosa increased and bacterial translocation rates of mesenteric lymphaden of TPNa group decreased significantly on day 1( P <0.05).On day 5,the level of NO and protein in intestinal mucosa increased significantly( P <0.05, P <0.01),and malondialdehyde decreased significantly( P <0.01). Conclusion:Acute pancreatitis may cause the damage of gut barrier;Arginine enriched TPN has protective effect on gut barrier of rats with acute pancreatitis.