| 正交试验法优选瘰疬膏水煎提取工艺 |
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| 引用本文： | 陈鹏英,杨柳. 正交试验法优选瘰疬膏水煎提取工艺[J]. 中国药师, 2014, 0(1): 84-86 |
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| 作者姓名： | 陈鹏英 杨柳 |
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| 作者单位： | 武汉市中医医院药学部 武汉 430014;武汉市中医医院药学部 武汉 430014 |
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| 基金项目： | 武汉市卫生局临床重点学科研究项目（编号：武卫［2005］294号） |
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| 摘 要： | 目的：优选瘰疬膏最佳水煎煮提取工艺.方法：设计3因素3水平9组试验,考察加水量、煎煮时间、提取次数对干膏得率影响;同时选取方中君药玄参的主要有效成分哈巴俄苷作为含量考察指标,采用HPLC法测哈巴俄苷的含量.结果：加水量8倍、煎煮时间1 h,提取3次工艺为干膏得率最高,哈巴俄苷的含量最高.结论：根据试验的结果,同时考虑成本,拟定以加水量8倍,煎煮时间1 h,煎煮3次为最佳提取工艺.
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| 关 键 词： | 瘰疬膏 正交试验 干膏得率 高效液相色谱法 哈巴俄苷 |
| 收稿时间： | 2013-07-03 |
| 修稿时间： | 2013-10-21 |
Optimization of Water Extraction Technique for Luoli Pastes |
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| Chen Pengying and Yang Liu. Optimization of Water Extraction Technique for Luoli Pastes[J]. China Pharmacist, 2014, 0(1): 84-86 |
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| Authors: | Chen Pengying and Yang Liu |
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| Affiliation: | Pharmacy Department of Wuhan Hospital of Traditional Chinese Medicine, Wuhan 430014, China;Pharmacy Department of Wuhan Hospital of Traditional Chinese Medicine, Wuhan 430014, China |
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| Abstract: | Objective： To investigate the water extraction technique for Luoli pastes. Methods ： According to the orthogonal exper- iment with three factors and three levels ,9 liquid samples by water extraction were prepared . The best water extraction technique was selected with the yield of extract and the content of harpagoside as the indices. Results ： The water extrac, tion with 8-fold water, 1 h boil- ing and 3 times extraction showed the highest extraction yield and harpagoside content. Conclusion： The optimal water extraction tech- nique is with 8-fold water,boiling lh and extracting 3 times. |
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| Keywords: | Luoli pastes Orthogonal experiment Extraction yield HPLC Harpagoside |
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