组蛋白去乙酰化酶1在子宫内膜异位症中的表达及意义

目的 研究组蛋白去乙酰化酶1(HDAC1)在子宫内膜异位症患者在位及异位内膜中的表达,探讨其在子宫内膜异位症发生、发展中的作用. 方法 应用免疫组织化学和免疫印迹法检测20例子宫内膜异位症患者在位内膜和异位内膜组织(研究组)中及20例子宫肌瘤患者的子宫内膜组织(对照组)HDAC1的表达情况. 结果 HDAC1阳性着色主要分布于子宫内膜上皮细胞和间质细胞的细胞核,在位内膜中HDAC1的表达强度明显高于对照组子宫内膜(P<0.01).免疫印迹检测提示,子宫内膜异位症在位内膜和异位内膜组织中HDAC1蛋白的相对表达量分别为2.67±0.69和2.55±1.36,显著高于对照组子宫内膜1.63±0.93(P<0.01,P<0.05);而在位内膜组与异位内膜组之间无统计学差异(P＞0.05). 结论 HDAC1在子宫内膜异位症在位和异位内膜组织中的高表达,可能在子宫内膜异位症的发生、发展中起重要作用.

Expression and significance of histone deacetylase 1 in endometriosis

Objective To investigate the expression of histone deacetylase1 (HDAC1) in the eutopic and ectopic endometrium of women with endometriosis. Methods The expression of HDAC1 in the eutopic and ectopic endometrium of 20 cases of women with endometriosis, and the expression of HDAC1 in the endometrium of 20 cases with hysteromyoma were mesured by immunohistochemical technique and Western blotting. Results HDAC1 was located in the nuclei of epithelial and stromal cells. The expression level of HDAC1 in eutopic endometrium was significantly higher than that in the control group (EM>P/EM><0.01). In Western blotting, semi quantitative analysis showed that the relative expression of HDAC1 protein in eutopic and ectopic endometrium were significantly higher than that in the control group (2.67±0.69；2.55±1.36 vs 1.63±0.93, EM>P/EM><0.01，EM>P/EM><0.05). But there was no significant difference between eutopic and ectopic endometrium (P>0.05). Conclusion The higher expression of HDAC1 in eutopic and ectopic endometrium with endometriosis indicates that it may play an import