p16,nm23-H1基因蛋白在膀胱癌的表达及与临床意义

目的探讨抑癌基因p16及肿瘤抑制基因nm23-H1在膀胱中的表达及临床意义.方法应用免疫组织化学s-p法检测18例正常膀胱组织中及38例膀胱移行细胞癌组织中p16及nm23-H1的表达.结果p16在正常组织、膀胱癌中阳性表达率分别为88.8%(16/18),47.4%(18/38),二者间有显著差异性(P＜0.01).nm23-H1在正常膀胱组织、膀胱癌中阳性表达率分别为83.3%(15/18),44.7%(17/38),二者间有显著差异(P＜0.01).提示p16,nm23-H1阳性表达率与膀胱移行细胞癌的临床分期、临床分级、肿瘤大小及是否转移有密切关系.结论p16、nm23-H1在膀胱癌中的阳性表达对抑制肿瘤生长、浸润和转移起重要作用,可作为膀胱癌患者预测转移和预后及判断膀胱移行细胞癌生物学行为的良好指标.

THE EXPREESION OF P16 AND NM-23H1 GENES PROTEIN IN BLADDER CARCINOMA AND CLINICAL SIGNIFICANCE

Objective:To study the expression and implication of p16 and nm23-H1 in human bladder carcinoma.Methods:Immunohistochemistry method was used to detect p16 and nm23-H1 expression in 18 noncancerous normal bladder tissues and 38 primary bladder carcinoma.Results:The positive expression rate of p16 in normal bladder tissues,primary bladder carcinoma tissues was 88.8%, 47.4%,respectively with significant difference (P＜0.01).The positive expression rate of nm23-H1 in normal bladder tissues and primary bladder carcinoma tissues was 83.5%,44.7%, respectively with significant difference(P＜0.01).The positive espression rate of p16 and nm23-H1 were closely correlated with clincopathological stages, pathlolgyical grades, tunor size and metastasis.Conclusions:Expression of p16 and nm23-H1 in bladder carcinoma play an important role in suppression of invasive growth and metastasis of tumor, might be a good indicator of predicting metastasis and porgnosis and evaluation the biological behavior of bladder carcinoma.