血清对氧磷酯酶1联合氧化指标检测在急性有机磷中毒的临床意义

目的探讨抗氧化酶及氧化应激压力在急性有机磷农药中毒（AOPP）中的意义。方法检测28例AOPP患者血清对氧磷酯酶1（PON1）、超氧化物歧化酶（SOD）和丙二醛（MDA）的水平并与36例健康对照组比较。结果28例AOPP患者PON1（58.9±6.4U/ml）和SOD（82.34±9.2U/ml）明显低于对照组PON1（140.1±8.9U/ml）和SOD（125.5±10.9U/m1）（P〈0.01）。MDA的水平（6.65±1.13nmol/ml）明显高于健康对照组（2.31±0.56nmol/ml）（P〈0.01）。中毒组PONl和MDA相关性分析后发现,PONl活性与MDA含量存在明显的负相关（r=-0.821,P〈0.01）。结论PONl等抗氧化酶活性下降,脂质发生过氧化反应,引发的氧化应激损伤在急性有机磷农药中毒病理生理机制中发挥重要作用。

Clinical Significance of Measurement of Serum Paraoxonase 1 and Antioxidative Enzymes Levels in Patients with Acute Organophosphorus Pesticide Poisoning

Objective To To explore the antioxidation of paraoxonase-1 （PON1）, superexide dismutase （SOD） and malondiadehyde （MDA） as markers of oxidative stress in patients with acute organophosphccus pesticide poisoning （AOPP）. Methods The levels of PON-1, SOD and MDA were measured in 28 cases and compared with 36 controls. Results The levelsof PON-1 and SOD in cases were significantly lower than in controls （P 〈 0.01）, while the level of MDA was significantly higher in cases. The activity of PON-1 was negatively correlated with the content of MDA in the patients with acute organophosphorus pesticide poisoning by correlation analysis （r= -0.821, P〈0.01）. Conclusions The oxidative damage caused by the decrease of antioxidative enzymes activity plays a pathophysiological role in the acute organphosphorus pesticide poisoning.