乌司他丁对脂多糖致大鼠急性肺损伤肺组织糖皮质激素受体的影响

目的探讨乌司他丁(UTI)对脂多糖(LPS)致大鼠急性肺损伤(ALI)组织糖皮质激素受体(GR)的影响,并了解其可能机制。方法清洁级成年雄性SD大鼠54只,随机分为3组:空白对照组(Con组,n=18),LPS组(n=18),脂多糖+乌司他丁组(LPS+UTI组,n=18)。各组又按照不同的时间点被分为4 h、8 h、12 h三个亚组。在各时间点处死大鼠,利用Western blot检测大鼠肺组织中GR的表达,实时荧光定量PCR检测GR m RNA的表达,同时酶联免疫吸附法(ELISA)检测肺组织匀浆液中肿瘤坏死因子(TNF)-α和白细胞介素(IL)-10的浓度以及肺组织病理学的变化。结果与对照组相比,LPS组GR蛋白表达在各时间点降低(P<0.05),LPS+UTI组GR蛋白在各时间点的表达水平高于LPS组,低于Con组(P<0.05);与对照组相比,LPS组GR m RNA表达降低(P<0.05),LPS+UTI组GR m RNA在各时间点的表达水平略高于LPS组(P>0.05),低于Con组(P<0.05);与对照组相比,LPS组TNF-α的表达在各时间点升高(P<0.05),LPS+UTI组TNF-α的表达水平在各时间点低于LPS组,高于Con组(P<0.05);与对照组相比,LPS组IL-10的表达在各时间点升高(P<0.05),LPS+UTI组IL-10的表达水平在各时间点高于LPS组(P<0.05)。组织病理学检查显示:LPS组肺组织见肺泡结构破坏,炎性细胞浸润,肺泡间隔增宽,出血,水肿,LPS+UTI组肺组织病理损害较LPS组明显减轻。结论 UTI能提高GR的表达;TNF-α和IL-10出现高峰的时间不同。

Effect of Ulinastatin on the expression of glucocorticoid receptor during the course of acute lung injury induced by Lipopolysaccharide endotoxin in rats

ObjectiveTo investigate the effect of Ulinastatin on the expression of glucocorticoid receptor during the course of acute lung injury induced by Lipopolysaccharide endotoxin in rats and to explore the underlyling mechanisms.Methods 54 male Sprague-Dawley rats were randomly divided into three groups with 18 mice each group： control group （Con group）, lipopolysaccharide group （LPS group）, lipopolysaccharide plus ulinastatin group （UTI group）. All groups were then divided into three subgroups of 4 h, 8 h, 12 h （n=6）. Rats were killed at indicated time points, and the protein and mRNA expression of GR in the lung were detected by Western blot and Real Time fluorescent quantitative PCR. The levels of TNF-α and IL-10 were also tested by ELISA. The pathology of lung tissue was evaluated by HE staining. Results Compared with control group, the protein expression levels of GR in LPS group were significantly lower. The protein expression levels of GR in UTI group were higher than those in LPS group, but lower than those in control group at all indicated time points. Compared with control group, theexpression levels of GR mRNA in LPS group were significantly lower at all indicated time points. The expression levels of GR mRNA in UTI group were slightly higher than those in LPS group. No significant differences were found between UTI and LPS group in the expression levels of GR mRNA at all time points. Compared with control group, the expression levels of TNF-α in LPS group were significantly higher at all time points. The expression levels of TNF-α in UTI group were lower than those in LPS group at all time point. Compared with control group, the expression levels of IL-10 in LPS group were higher at all time points. In UTI group, the expression levels of IL-10 were higher than those in LPS group at all time points. Lung hispathology： necrotic abscission cells increased in alveolar space, inflammatory cells infiltration and lung interstitial edema appeared in LPS group. However, pathological changes i