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| 用McAb-AST对犬内脏利什曼病早期诊断的初步研究 | |
| 引用本文： | 王雅静,胡孝素,林芳清. 用McAb-AST对犬内脏利什曼病早期诊断的初步研究[J]. 寄生虫病与感染性疾病, 1993, 0(2) |
| 作者姓名： | 王雅静 胡孝素 林芳清 |
| 作者单位： | 华西医科大学寄生虫学研究室，华西医科大学寄生虫学研究室，华西医科大学寄生虫学研究室 成都 610041，成都 610041，成都 610041 |
| 基金项目： | 卫生部青年科学研究基金 |
| 摘 要： | 本文报道实验感染杜氏利什曼原虫小犬10只,在感染前及咸感后5、12、20、40d,分别收集血清,用McAb-AST检测血清内循环抗原。试验结果可见小犬感染后5d即出现阳性反应,表明已可检测出其血清内循环抗原,并随着时间的加长,阳性反应程度有所增加。初步建立了犬利什曼病的一种新的早期诊断方法,简便易行,易于推广应用。 |
| 关 键 词： | 犬利什曼病 杜氏利什曼原虫 单克隆抗体 早期诊断 |
EARLY DIAGNOSIS OF CANINE LELSHMANIASIS BY McAb-AST |
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| Wang Yajing,Hu Xiaosu,Lin Fangqing Laboratory of Parasitology,West China University of Medical Sciences,Chengdu .. EARLY DIAGNOSIS OF CANINE LELSHMANIASIS BY McAb-AST[J]. Parastoses and Infectious Diseases, 1993, 0(2) | |
| Authors: | Wang Yajing Hu Xiaosu Lin Fangqing Laboratory of Parasitology West China University of Medical Sciences Chengdu . |
| Affiliation: | Wang Yajing,Hu Xiaosu,Lin Fangqing Laboratory of Parasitology,West China University of Medical Sciences,Chengdu 610041. |
| Abstract: | In order to develop a method for early diagnosis of canine leishmaniasis, McAb -G_(10)-A_4 was used to detect L. donovani circulating antigen by McAb-AST. Sera were taken from 10 dogs experimentally infected by L. donovani amastigotes before infection and 5,12,20,40 days after infection. The results showed that the positive reaction appeared as early as 5 days after infection and the circulating antigen in the serum could be detected since then. As the time went on, the in- tensity of positive reaction increased. In control sera from 50 dogs in non-endemic areas, only 1 dog showed positive. Thus, a new method of early diagnosis of canine leishmaniasis was devel- oped. It is simple and convenient, and can be easily applied in the field conditions. |
| Keywords: | Canine leishmaniasis Leishmania donovani monoclonal antibody early diagnosis |
| 本文献已被 CNKI 等数据库收录！ | |