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| 用聚合酶链反应技术探测人载脂蛋白AI基因多态性 | |
| 引用本文： | 韩琴琴,陈斌,翁国璐,陈灏珠. 用聚合酶链反应技术探测人载脂蛋白AI基因多态性[J]. 复旦学报(医学版), 1993, 0(4) |
| 作者姓名： | 韩琴琴 陈斌 翁国璐 陈灏珠 |
| 作者单位： | 上海医科大学中山医院，上海市心血管研究所，上海医科大学中山医院，上海市心血管研究所，上海医科大学中山医院，上海市心血管研究所，上海医科大学中山医院，上海市心血管研究所 |
| 基金项目： | 国家自然科学基金(3880412) |
| 摘 要： | 用聚合酶链反应(PCR)技术扩增载脂蛋白(Apo)AⅠ基因第3个内含子中包括MspⅠ多态位点在内的总长307bp DNA片段,观察中国汉族健康人、高脂血症和冠心病患者Apo AⅠ基因多态性。结果表明,中国汉族人种Apo AⅠ基因Msp Ⅰ多态性与白种人不同,M_2等位基因属常见等位基因,而白种人为罕见等位基因。高脂血症和冠心病患者M_2等位基因频率与健康人无差异。 |
| 关 键 词： | 载脂蛋白AI基因 聚合酶链反应技术 基因多态性 高脂血症 冠心病 |
POLYMORPHISM OF APOLIPOPROTEIN RI GENE STUDIED WITH POLYMERASE CHAIN REACTION |
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| Han Qin-Qin,Chen Bing,Weng Guo-Lu,Chen Hao-Zhu,. POLYMORPHISM OF APOLIPOPROTEIN RI GENE STUDIED WITH POLYMERASE CHAIN REACTION[J]. Fudan University Journal of Medical Sciences, 1993, 0(4) | |
| Authors: | Han Qin-Qin Chen Bing Weng Guo-Lu Chen Hao-Zhu |
| Abstract: | Using the polymerase chain reaction (PCR) to amplfy 807 bp fragment of the third intron of apolipoprotein AI gene which contains the Msp-I polymorphism site, the frequency of DNA polymorphisms in apolipoprotein AI gene loci in normal Chinese and patient with hyperlipidemias or coronary disease was studied. The results showed that occurrence of M2 allele is more common in the Chinese than that rarely in Caucasians. No difference was found between the frequency of M2 allele in healthy subjects and patients with hyperlipidemias or coronary artery disease. |
| Keywords: | apolipoprotein AI polymerase chain reaction polymorphisms hyperlipidemias coronary artery disease |
| 本文献已被 CNKI 等数据库收录！ | |