丰城鸡血藤活性成分HPLC指纹图谱研究

目的：建立丰城鸡血藤和鸡血藤黄酮类化合物的HPLC指纹图谱。方法：采用反相高效液相色谱法，选用Hypersil ODS-C18柱（250mm×4．6mm，5μm）色谱柱；流动相为乙腈-0．1％甲酸溶液梯度洗脱；分析时间：75min；检测波长：249nm。结果：建立了丰城鸡血藤和鸡血藤药材黄酮类化合物的HPLC指纹图谱，标定了鸡血藤药材的共有指纹峰，方法学考察结果符合指纹图谱技术要求。结论：方法稳定、可靠、重复性好，可为丰城鸡血藤药材质量控制标准的制定提供参考。

HPLC Fingerprint Spectrum of Millettia nitida Benth var hirsutissima Z Wei

Objective：To estalbish the HPLC fingerprint spectrum of MiUettia nitida Benth var hirsutissima Z Wei. Methods： The RP-FIPLC method was used; Hypersil ODS-C18 column（250 mm× 4.6 mm, 5 μm） was employed; the acetonitrile-0.1% Meth acid was used as mobile phase;analytic time was 75 mins, and detective wavelength was 249 ima. Result： The HPLC fingerprint spectrum of Millettia nitida Benth vat hirsutissima Z Wei was set up. The conmon peaks in different sources were pointed out. The results of method validation met technical standard of fingerprints. Conclusion：The method is stable and reliable with a good reproducibility and provides a reference standard for the quality control of Millettia nitida Benth. vat. hirsutissima Z. Wei.