三氧化二砷阻滞头颈部鳞状细胞癌细胞周期调控肿瘤细胞凋亡的研究

目的:探究三氧化二砷(arsenic trioxide,ATO)对头颈部鳞状细胞癌细胞周期变化以及细胞凋亡的影响。方法:对舌鳞状细胞癌SCC25细胞给予不同浓度的ATO处理后,采用台盼蓝染色检测活细胞数以及MTT观察细胞增殖活性的变化,TUNEL检测细胞凋亡,流式细胞术(flow cytometry,FCM)检测细胞周期变化;Western Blot检测pCDK2以及pHH3表达的变化。结果:台盼蓝染色和MTT结果显示,ATO抑制SCC25细胞的活性且具有浓度依耐性。TUNEL结果显示,ATO可促进SCC25细胞凋亡。FCM结果显示,ATO可减少G1/G0期的肿瘤细胞的比例,增加G2/M期细胞比例。Western Blot结果表明,随着ATO浓度的增加,pHH3表达量增多,而pCDK2表达量减少。结论:ATO对SCC25细胞的增殖具有抑制作用,对SCC25胞的凋亡具有促进作用,其机制可能是通过ATO介导SCC25细胞发生G2/M期细胞周期阻滞而实现的。

Apoptotic cell death by arsenic trioxide through mediating cell cycle arrest in human HNSCC cells

Objective:To investigate the effect of arsenic trioxide(ATO)on the proliferation,apoptosis and cell cycle of head and neck squamous cell carcinoma cells.Methods:SCC25 was treated by different concentrations of ATO,and then using Trypan blue staining assay to test the counter of viable cells.MTT assays and TUNEL method were applied to assess cell proliferation and apoptosis.Flow cytometry(FCM)analysis was adopted to examine cell cycle.And the changes on the expressions of pCDK2 and pHH3 were detected by Western Blot.Results:The results of MTT and Trypan blue stain assay showed that different concentration of ATO inhibited the proliferation of SCC25 cells.According to the TUNEL result,ATO with the high concentration promoted apoptosis of SCC25 cells.FCM results showed that ATO reduced the proportion of tumor cells in G1/G0 phase and increased the proportion in G2/M phase.Western Blot results showed that with the concentration of ATO gradually increased,the expression of pHH3 increased,while the expression of pCDK2 decreased.Conclusion:ATO can inhibit the proliferation and promote the apoptosis of SCC25,which may be achieved by blocking the cell cycle arrest of SCC25 cells at G2/M phase.