LCRG1 suppresses tumor growth in vivo by liposome-mediated gene transfer

Objective: To investigate whether LCRG1 (Laryngeal Carcinoma Related Gene 1) has tumor suppressor function. Methods: The recombinant plasmid pcDNA3.1(+)/LCRG1 was successfully constructed. The biological effects of LCRG1 on Hep-2 cell line were studied by cell transfection, cell growth observation colony formation analysis and tumorigenicity experiments. Results: The LCRG1 gene potently inhibited tumorgenesis in vitro and in vivo, as showed by dramatic growth arrest observed in cell growth analysis and suppression of anchorage-independent growth and tumorigenicity in nude mice. Conclusion: Our results suggested that LCRG1 may be a candidate of tumor suppressor gene. Foundation item: This work was supported by the National Natural Science Foundation of China(No. 39900052) and a grant from the Ministry of Health of PR China (No. 98-1-118). Biography: Li You-jun(1972–), doctor of medicine, assistant professor, Xiangya Medical School, Central South University, majors in pathology and pathophysiology.