口腔粘膜癌前病变及鳞癌角蛋白表达的免疫组化研究

目的 研究口腔粘膜标本中角蛋白的表达与分布。方法 利用多克隆抗角蛋白抗体Ａ５７５，采用免疫组化技术。结果 从正常口腔粘膜，轻、中、重度异常增生到鳞癌角蛋白表达显著增强，Ａ５７５在所有鳞癌标本中均为阳性，但染色强度深浅不一，且随鳞癌分化程度降低而降低，染色弱的病例其临床分期高，更易发生淋巴结转移。结论角蛋白表达的增强在强人上皮细胞中的致癌基因上起作用，这种强化的积累将加速癌前疾病的进展，因此，Ａ５７

Immunohistochemical study of oral mucosa precancerous lesion and expression of cytokeratin of squamous cell carcinoma]

OBJECTIVE:The aim of this study was to investigate the expression and distribution of cytokeratin in oral mucosa. METHODS: Polyclonal cytokeratin antibody A(575) and ABC immunohistochemical technique were used. RESULTS: A tendency of progressive increase of cytokeratin expression was demonstrated from normal mucosa,mild moderate and severe dysplasia to squamous cell carcinoma. A notable overexpression was found in moderate,severe dysplasia and squamous cell carcinoma.The poor-differentiatin was related to the low reaction of A(575).The cases of strong reaction of A(575)were more advanced stages and metastasized to lymph nodes frquently. CONCLUSION: The overexpression of cytokeratin could play a role in potentiation the effects of genetic factors and activate oncogenes in the affected cells.The cumulative effects of the potentiation would be accelerate the progression of precancerous conditions.The reaction of A(575) was correlated with clinical stages and lymph node metastasis.