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| NOD小鼠CD4^＋CD25^＋调节性T细胞功能变化研究 | |
| 引用本文： | 李卫鹏,申勇,胡永全,史桂英,王福庆.NOD小鼠CD4^＋CD25^＋调节性T细胞功能变化研究[J].蚌埠医学院学报,2008,33(6):743-746. |
| 作者姓名： | 李卫鹏 申勇 胡永全 史桂英 王福庆 |
| 作者单位： | 1. 蚌埠医学院第一附属医院,核医学科,安徽,蚌埠,233004 2. 上海交通大学医学院,细胞生物学教研室,上海,200025 3. 上海交通大学医学院,上海市免疫学研究所,上海,200025 |
| 摘 要： | 目的：观察NOD小鼠CD4^＋CD25^＋调节性T细胞的功能变化。方法：流式细胞仪分析NOD小鼠胰腺引流淋巴结（PLN）CD4^＋CD25^＋T细胞频率,CD4^＋CD25^＋T细胞中FoxP3^＋细胞的比例,CD4^＋CD25^＋FoxP3^＋T细胞的FoxP3平均荧光强度（MFI）;检测CD4^＋CD25^＋调节性T细胞的抑制功能。结果：在不同的年龄阶段,CD4^＋CD25^＋调节性T细胞数量未发生明显变化,而随时间的推移,CD4^＋CD25^＋调节性T细胞中FoxP3表达水平降低,CD4^＋CD25^＋调节性T细胞的功能也下降。结论：CD4^＋CD25^＋调节性T细胞的抑制功能降低可能是NOD小鼠糖尿病发作的根本原因。 |
| 关 键 词： | 糖尿病 实验性 免疫 细胞 平均荧光强度 转录因子FOXP3 小鼠 |
Changes of function of CD4~+CD25~+ T regulatory cells in NOD mice |
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| LI Wei-peng,SHEN Yong,HU Yong-quan,SHI Gui-ying,WANG Fu-qing.Changes of function of CD4~+CD25~+ T regulatory cells in NOD mice[J].Journal of Bengbu Medical College,2008,33(6):743-746. | |
| Authors: | LI Wei-peng SHEN Yong HU Yong-quan SHI Gui-ying WANG Fu-qing |
| Institution: | LI Wei-peng1,SHEN Yong1,HU Yong-quan1,SHI Gui-ying2,WANG Fu-qing3(1.Department of Nuclear Medicine,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,2.Department of Cell Biology,3.Shanghai Institute of Immunology,Shanghai Jiaotong University of Medicine,Shanghai 200025,China) |
| Abstract: | Objective:To investigate the function of CD4+CD25+ T regulatory(CD4+CD25+ Treg)cells in NOD mice.Methods:The frequency of CD4+CD25+T cells from pancreatic lympho node(PLN)was analysed,the percentages change over time in the proportion of NOD CD4+ CD25+ T cells expressing Foxp3 were observed,mean fluorescent intensity(MFI)of Foxp3 in CD4+ CD25+ FoxP3+T cells was assayed,and in vitro suppression assay was performed.Results:No percentages change over time in the proportion of NOD CD4+ CD25+ T cells expressing ... |
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