尼美舒利对大鼠实验性牙周炎防治作用的评价

目的： 评价尼美舒利对实验性牙周炎的防治效果，探讨前列腺素与牙周炎发病的关系，为牙周炎临床治疗的联合用药提供依据。方法： Sprague-Dawly大鼠120只，随机分成4组。正常对照组及各实验组分别于4、5和8周末分批处死动物。在处死动物前测量各时点的牙龈指数，通过组织学方法观察各组动物牙周组织的病理学改变，用Tiger细胞图像分析仪测量牙周附着丧失情况。结果： （1）大鼠经丝线结扎4周后成功复制出牙周炎模型。（2）实验性牙周炎组，4周时牙龈指数明显高于正常对照组(P<0.01)，附着丧失明显高于正常对照组(P<0.01)。组织学观察到牙周组织出现明显的病理改变，并且牙周组织破坏程度随着时间的推移逐渐加重。（3）尼美舒利预防组牙龈指数和附着丧失明显低于牙周炎组(P<0.01)，组织学观察到牙周组织的炎症较轻，牙槽骨未见明显破坏吸收。（4）在实验性牙周炎形成后，尼美舒利治疗组自第5周开始给予尼美舒利治疗，5周和8周末时牙龈指数和附着丧失明显低于牙周炎组(P<0.01)，8周时牙龈指数和附着丧失降低尤为明显。结论： 预防性给予尼美舒利，可有效阻止实验性牙周炎的发展；在牙周炎发生后，给予尼美舒利治疗，能有效降低牙周组织的炎症和破坏程度。

Effect of nimesulide on ligature-induced periodontitis in rats

AIM: The purpose of this study was to observe the effect of nimesulide on periodontitis. METHODS: The gingival index (GI) was measured before the rats were sacrificed at the ends of week 4, 5 and 8. The periodontal tissues were stained with hematoxylin and eosin. Histological changes were observed by microscope. The periodontal attachment loss (AL) was measured by Tiger cell image analyzer. RESULTS: (1) Experimental periodontitis was successfully induced in rats by placing a piece of 3/0 braided silk around the cervix of the lower incisors at week 4 after the ligature. (2) In ligature-induced periodontitis group, at week 4 after the ligature, the GI and AL were significantly higher than those in control group (P<0.01). The histopathologic changes of periodontium in periodontitis group showed obvious inflammation, and the severity of destruction for periodontium was increased as time passed. (3) In the nimesulide prevention group, the GI and AL were significantly lower than those in periodontitis group (P<0.01). The histopathologic examination showed less inflammatory responses, and no obvious alveolar bone resorption was observed. (4) In the nimesulide treatment group, the GI and AL were significantly lower than those in periodontitis group at the end of week 5 and 8 after the ligature (P<0.01).CONCLUSION: (1) In ligature-induced periodontitis, nimesulide inhibits effectively its progress. (2) In the developing periodontitis, a significant improvement is observed in GI and AL following the treatment with nimesulide.