人硫氧还蛋白在兔脑缺血再灌注损伤中的作用

目的通过CT灌注及测量脑水肿程度,观察人硫氧还蛋白(RX)对局灶性兔脑缺血/再灌注损伤的保护作用.方法采用线栓法制成一侧兔脑缺血/再灌注模型(栓塞6 h,再灌注18 h),将25只雄性新西兰白兔随机分成假手术组(5只)、缺血/再灌注组(10只)和缺血/再灌注 RX组(10只),后者给予RX(0.75 mg/kg体重),其他组给予等容积的生理盐水.分别于梗死后6 h及再灌注后18 h做CT灌注图像,计算脑梗死面积占同侧同层大脑半球面积的百分比;测量脑组织含水量.结果脑缺血/再灌注后,应用RX治疗,脑梗死面积显著减少,脑水肿减轻.结论重组RX对脑缺血/再灌注损伤有显著的治疗作用.

Effect of human Thioredoxin on cerebral ischemia/reperfusion injury by perfusion CT and measurement of edema

Objective To investigate the protective function of human Thioredoxin (hTRX) on cerebral ischemia/reperfusion injury of rabbit by perfusion CT and measurement of the degree of edema. Methods Unilateral cerebral ischemia/reperfusion models of rabbit were obtained by the intraluminal filament technique (embolized for 6 hours followed by reperfusion for 18 hours). Total 25 rabbits were randomly divided into sham operation group (n=5), ischemia/reperfusion group (n=10), and recombinant hTRX protein treatment group (n=10). The recombinant hTRX protein was administered to Group I/R hTRX (0.75 mg/kg body weight), and the same volume of 0.9% sodium chloride was given to the other two groups. Perfusion CT scans were performed respectively 6 hours after ischmia and 18 hours after reperfusion, and the cerebral ischemia areas were observed, then the percents of ischmia areas account for the same cerebral areas in the same slice were calculated. Water content of the brain tissue was measured. Results After cerebral ischemia/reperfusion, the ischemia areas were significantly reduced (average reduced 30.6%), and the degree of edema was significantly lightened by using hTRX. Conclusion The recombinant hTRX protein has good effect on the treatment of cerebral ischemia/reperfusion injury.