|
|||||
|
|
| 人类滤泡树突状细胞的分离 | |
| 引用本文： | 姜拥军,范霞,康辉,Tylor Thacker. 人类滤泡树突状细胞的分离[J]. 中国免疫学杂志, 2001, 17(3): 152-154 |
| 作者姓名： | 姜拥军 范霞 康辉 Tylor Thacker |
| 作者单位： | 1. 中国医科大学附属第一医院艾滋病研究室，沈阳110001 2. Brigham Young University,UT,USA 84602 |
| 摘 要： | 为探讨人类滤泡树突细胞（FDC）在HIV致病机制中的作用，摸索分离出提取人FDC的最佳方法。方法：用胶原酶IV和DNA酶消化人偏桃体，Percoll连续梯度分层液分离含有FDC的细胞层，经MACS分选，得到富含FDC的细胞，γ－射线将混有的B细胞、T细胞照射坏死。结果：流氏细胞仪分析FDC纯度约为70.7％，^3H掺入法实验证明分离提取的细胞具有FDC细胞的活化，IL－6和IL－2的测定结果显示分离提取的细胞具有FDC的特征。结论：此方法为一种行之有效的FDC分离提取方法，为进一步研究FDC在HIV及肿瘤致病机理中的作用打下基础。 |
| 关 键 词： | 人类滤泡树突状细胞 分离方法 FDC 艾滋病 发病机制 |
| 文章编号： | 1000-484X(2001)03-0152-03 |
Isolation of humanfollicular dendritic cell |
|
| JIANG Yong Jun,FAN Xia,KANG Hui et al AIDS Research Lab,the First Affiliated Hospital,China Medical University,Shenyang. Isolation of humanfollicular dendritic cell[J]. Chinese Journal of Immunology, 2001, 17(3): 152-154 | |
| Authors: | JIANG Yong Jun FAN Xia KANG Hui et al AIDS Research Lab the First Affiliated Hospital China Medical University Shenyang |
| Affiliation: | JIANG Yong Jun,FAN Xia,KANG Hui et al AIDS Research Lab,the First Affiliated Hospital,China Medical University,Shenyang 110001 |
| Abstract: | Objective: In order to study the role of FDC in HIV pathogenesis,an optimal method for isolating FDC was studied.Methods: Tonsiles were digested with col lagenase IV and Dnase I , and cellular layer including FDC was obtained by Perco ll. Purified FDC was yielded by MACS sorting ,and contaminated B and T cells w e re irradiated by γ-ray. Results:The purity of FDC analyzed with flow cytometer is about 70.72%, the thymidine incorporation showed that FDC cells with activi ty were yielded and the results of IL-6 & IL-2 determination indicated that isol ate d cells were with FDC characteristics.Conclusion: This method is a practical met hod for isolating FDC for further investigation of FDC in HIV and neoplastic pa thogenesis. |
| Keywords: | Human follicular dendritic cell Isolating method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |