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Complete RNA1 sequences of two UK isolates of barley mild mosaic virus: a wild-type fungus-transmissible isolate and a non-fungus-transmissible derivative
Authors:Ellen Peerenboom  Ewen J Cartwright  Ian Foulds  Michael J Adams  Rebecca Stratford  Arie Rosner  Hans-Henning Steinbiss  John F Antoniw
Affiliation:

a Max-Planck-Institut fur Züchtungsforschung, Abt. Genetische Grundlagen der Pflanzenzuchtung, Carl von-Linne-Weg 10, 50829 Köln, Germany

b Crop and Disease Management Department, IACR-Rothamsted, Harpenden, Herts AL5 2JQ, UK

c PBI Cambridge, Maris Lane, Trumpington, Cambridge CB2 2LQ, UK

d Virology Department, Volcani Centre, Bet-Dagan 50250, Israel

Abstract:The complete RNA1 sequences of two isolates (fungus transmissible and non-fungus transmissible) of barley mild mosaic virus (BaMMV) were obtained. The two isolates' RNA1 sequences had very high sequence identity (99.3%), and of the 15 amino acid differences (out of 2258) between the putative polyproteins, 11 were conservative and unlikely to affect the structure or function of the protein. The remaining amino acid differences were thought unlikely to affect fungus transmission because they occur in the CI- and NIb-coding regions. This strongly suggests that the P73 protein of RNA2 (which has a 364-aa deletion in the non-fungus-transmissible isolate) is involved in fungus transmission of BaMMV.
Keywords:mosaic virus   rna sequence   virus rna   virus protein
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