乌司他丁对肠源性脓毒症大鼠D-二聚体和蛋白C系统的影响

目的：观察乌司他丁（ulinastatin，UTI）对肠源性脓毒症大鼠D-二聚体（DD）、蛋白C（PS）系统的影响。方法：将Wistar大鼠随机分为4组，假手术组（SH组，n=36）、脓毒症组（SEP组，n=30）、UTI3h给药组（UTI3h组。n=30）和12h给药组（UTI12h组，n=18）。利用经典盲肠结扎穿孔法建立肠源性脓毒症大鼠模型，分别于正常对照组及制模后6、12、24、48及72h组心脏取血测定DD、PC和蛋白S（PS）。结果：与SH组相比，SEP组大鼠DD在造模后逐渐升高，PC、PS含量降低，差异有显著性（P〈0．05）。与SEP组相比，UTI3h,UTI12h组大鼠的DD降低，PC含量下降趋势减弱，差异有显著性（P〈0．05）。结论：UTI能有效改善脓毒症期间的抗凝抑制和继发性纤溶亢进。

Effects of ulinastatin on serum D-dimer and protein C system in rats with severe gut-origin sepsis

Objective To observe the effects of ulinastatin (UTI) on serum D-dimer (D-D) and protein C (PC) system in rats with severe gut-origin sepsis. Methods One hundred and fourteen Wistar rats were randomly divided into four groups: sham group (n=36), sepsis group (n=30), UTI3h group (n=30), and UTI12h group (n=18). The rat model of gut-origin sepsis was established by classic cecal ligation and puncture (CLP). Serum levels of D-D, PC, and protein S(PS) were detected in the normal and experimental rats 6, 12, 24, 48, and 72 h after CLP. Results The D-D level was higher but the levels of PC and PS were significantly lower in the sepsis group than in the sham group (P < 0.05). As compared with those in the sepsis group, D-D was decreased and PC was increased markedly in the UTI3h or UTI12h group (P < 0.05). Conclusion Ulinastatin can effectively improve the suppression of anticoagulation and resultant excessive fibrinolysis.