重力打击式小鼠外伤性视神经病变模型的建立及外伤性视神经病变发病机制的探讨

目的 通过建立重力打击式小鼠外伤性视神经病变模型探讨外伤性视神经病变的发病机制.方法 选取SPF级C57BL/6J成熟小鼠48只(96眼),均取小鼠左眼为实验眼进行造模,右眼为对照眼不做处理.采用重力打击建立小鼠外伤性视神经病变模型,造模后第1、3、5、7天对小鼠进行观察,每个时间点取6只小鼠进行闪光视觉诱发电位检查、...

Establishment of the gravity strike model of mouse traumatic optic neuropathy and analysis on its mechanisms

Objective　To investigate the pathogenesis of traumatic optic neuropathy by establishing a gravity strike model of traumatic optic neuropathy in mice.Methods　A total of 48 mature C57BL/6J mice (96 eyes) in specific pathogen free (SPF) level were selected. Their left and right eyes were considered as the experimental and control groups, respectively. The traumatic optic neuropathy model of mice was established by a gravity strike on the left eye. Mice were observed on the 1st, 3rd, 5th and 7th day after modeling. At each time point, 6 mice were taken for flash visual evoked potential (F-VEP) examination and HE staining of retinal tissues. Expression levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in the experimental and control retina were detected by RT-PCR.Results　F-VEP results showed that a significant negative wave was detected in both the control eye and the experimental eye, which was named as N1 wave in this experiment. On day 1, 3, 5 and 7 of the experiment, the amplitude of F-VEP in experimental eyes was significantly lower than that in control eyes (all P<0.001). After modeling, the retinal of the control eye had a clear structure, well orderly arranged retinal ganglion cells, and clear nuclei with smooth and complete nuclear membrane. On the contrary, slightly thickened retina, the formation of intercellular vacuoles and disordered arrangement of ganglion cells in the retinal ganglion cell layer, and the loss of ganglion nuclei was observed on the 7th day of experimental eye. Expression levels of IL-1β and TNF-α in the experimental retina of was significantly lower than that of controls at the 1st, 3rd, 5th and 7th day after modeling (all P<0.05), which were downregulated with the prolongation of modeling (all P<0.05).Conclusion　The gravity strike model of traumatic optic neuropathy in mice established in this study is simple to operate and has a better clinical simulation effect. In this model, IL-1β and TNF-α were upregulated in the retina of mice with traumatic optic neuropathy.