敲低PRMT5基因表达对胃癌细胞增殖、侵袭和凋亡的影响及其机制

目的 观察蛋白质精氨酸甲基转移酶5(PRMT5)在胃癌细胞中的表达,探讨敲低PRMT5表达水平后对胃癌细胞生物学行为的影响.方法 Western blot检测胃癌细胞系MGC803、SGC7901、MKN45和人胃黏膜上皮细胞GES-1中PRMT5蛋白表达水平,转染siRNA1和siRNA2质粒敲低PRMT5的表达水平...

Effect of PRMT5 Expression Knockdown on Proliferation,Invasion and Apoptosis ofGastric Cancer Cells and Its Mechanism

Objective To observe the expression level of PRMT5 in gastric cancer cells, and explore theeffect of knocking down the expression level of PRMT5 on the biological behavior of gastric cancer cells.Methods Western blot was used to detect the expression of PRMT5 protein in gastric cancer cell linesMGC803, SGC7901, MKN45 and human gastric epithelial cells GES-1. siRNA1 and siRNA2 plasmids weretransfected to knock down the expression of PRMT5. Cell proliferation and apoptosis assay, transwell assaywere used to detect cell proliferation, apoptosis and invasion abilities, respectively. The protein expressionlevels of β-catenin, cyclin D1 and Bax were detected. Results Compared with GES-1 cells, PRMT5 proteinexpression levels increased in MGC803, SGC7901 and MKN45 cells (P<0.001). After knocking down theexpression of PRMT5, the cell proliferation and invasion abilities were weakened, the apoptosis rate increased(P<0.05), the expression of β-catenin and Cyclin D1 protein decreased, and the expression of Bax proteinincreased (P<0.001). Conclusion The expression level of PRMT5 is increased in gastric cancer cells.Knockdown of PRMT5 expression level could inhibit cell proliferation, invasion and promote cell apoptosisvia reducing Wnt/β-catenin signaling pathway.