白黎芦醇体外对多房棘球蚴原头节和微囊的作用研究

目的 研究白藜芦醇(Resveratrol,RES)体外对多房棘球蚴原头节(Protoscoleces,PSCs)和微囊的作用效果,为多房棘球蚴病的临床用药提供新的依据.方法 PSCs体外经不同浓度(5、10、20、40、80、100、200和400 μmol/L)的RES作用7 d,采用台盼蓝染色观察其活力与形态变化...

Effect of resveratrol against Echinococcus multilocularis protoscoleces and microcysts in vitro

To investigate the effect of resveratrol (RES) against protoscoleces (PSCs) and microcysts of Echinococcus multilocularis in vitro, providing the new references for drugs application to treat alveolar echinococcosis. The vitality and morphological changes in PSCs exposed to RES at different concentrations (5, 10, 20, 40, 80, 100, 200 or 400 μmol/L) for 7 days in vitro were investigated by staining with 0.4% trypan blue, and the ultrastructural alterations were imaged by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively; alkaline phosphatase (ALP) activity in culture supernatants was measured using chemiluminescence method. Further, the viability and morphology of E. multilocularis microcysts after treatment with RES for 14 days in vitro were investigated under a light microscopy, and then ALP activity in culture supernatant was measured using chemiluminescence method. The mortality of E. multilocularis PSCs increased with a dose- and time-dependent manner after treatment with RES at 5 to 400 μmol/L during 7 days in vitro, accompanying with obvious hooks loss. The mortality of PSCs reached 100% after treatment with 40 μmol/L RES for 6 days, and the ultrastructure of suckers, hooks and tegument were destroyed, however, under the same conditions, the mortality of PSCs was only (13.25±1.41)% and (6±0.71)% after treatment with ABZ-SO and dimethye sulfoxide (DMSO), respectively. The ALP activity in culture supernatant of PSCs significantly increased with a dose-dependent manner (F=36.94, P<0.001) on 6^th day at different concentrations of RES. When treated with 20 or 40 μmol/L RES, the ALP activity was (2.72±0.24) U/L and (2.95±0.10) U/L, respectively, which posed significant differences in comparison with DMSO treatment (1.97±0.18) U/L (t=2.94, P=0.026 and t=7.066, P=0.000). In addition, after treatment with 40 μmol/L RES for 14 days, light transmittance in E. multilocularis microcysts deceased observably, and the germinal layer was shrunk and separated from laminated layer. Meanwhile, ALP activity (2.74±0.15) U/L in RES group was observably higher than that in DMSO group (2.08±0.09) U/L (t=3.83, P=0.019), but there was no significant difference between ABZ-SO group (2.29±0.14) U/L and DMSO group (t=1.271, P=0.273). Resveratrol could obviously kill E. multilocularis PSCs and microcysts in vitro, which may be a potential candidate drug for treatment of alveolar echinococcosis.