牛磺酸对乙醇醛细胞毒性的抑制作用

目的：研究牛磺酸对乙醇醛所致细胞毒性的抑制作用。 方法：采用单细胞凝胶电泳技术（彗星实验）检测牛磺酸（50 mmol•L^-1）对乙醇醛（GA, 0.1 mmol•L^-1）所致小鼠脾淋巴细胞DNA损伤的抑制作用，并用2,4-二硝基苯肼法定量检测牛磺酸对反应体系中乙醇醛含量的影响，同时设乙醇醛阳性对照组和PBS阴性对照组。 结果：在0.1 mmol•L^-1乙醇醛溶液中加入50 mmol•L^-1牛磺酸后，小鼠脾淋巴细胞DNA的损伤率较阳性对照组明显降低（P<0.01），彗星实验的迁移距离（尾长）也显著缩短（P<0.01），牛磺酸呈剂量依赖性明显降低反应体系中乙醇醛的含量。 结论：牛磺酸对乙醇醛的细胞毒性有明显抑制作用。

Inhibitory effects of Taurine on glycolaldehyde-induced cellular toxicity

Objective To investigate the inhibitory effects of Taurine on the cellular toxicity induced by glycolaldehyde. Methods The inhibitory effects of Taurine (50 mmol·L~ -1) on glycolaldehyde (0.1 mmol·L~ -1) induced DNA damage in spleen lymphocytes of mice were studied in vitro using single cell gel electrophoresis (comet assay),and 2,4-dinitrophenylhydrazine (DNPH) measurement was used to detect quantitatively the effects of Taurine on carbonyl compound contents in a reactive system. Results After 50 mmol·L~ -1 Taurine was added to the spleen lymphocyte suspension containing 0.1 mmol·L~ -1 glycolaldehyde, the cellular DNA damage rates were markedly decreased (P<0.01) and the migration distances of DNA were significantly reduced (P<0.01) as compared with positive control group, and Taurine reduced the carbonyl compound contents in a reactive system in a dose-dependent manner. Conclusion Taurine can significantly inhibit the cellular toxicity induced by glycolaldehyde.