祛湿化瘀方对单纯高脂饮食诱导的大鼠脂肪肝的防治作用

目的：观察中药祛湿化瘀方治疗非酒精性脂肪肝的疗效。方法：SD雄性大鼠36只，SPF级，随机取5只为正常组。其余大鼠以高脂饲料（普通饲料＋2％胆固醇＋10％猪油）饲养12周诱导脂肪肝。期间在第7周起，按随机号分模型组（11只）、祛湿化瘀方组（10只）及对照药物甘乐组（10只），分别予以相应药物或蒸馏水灌胃6周。取材后观察肝组织病理（HE染色和油红染色）、电镜超微结构；肝组织甘油三酯（TG）、游离脂肪酸（FFA）含量；血清ALT和AST活性、胰岛素抵抗指数、血脂等变化。结果：模型组肝体肥大，肝组织出现显著的脂肪变性，电镜可见肝细胞内存在大量脂滴，肝组织中TG含量达正常组5．2倍、FFA含量显著升高，但血脂含量和ALT、AST活性及胰岛素抵抗指数无显著变化。祛湿化瘀方组可显著改善肝组织病理变化，显著降低肝组织TG和FFA含量（其均值分别为模型组的54．5％和72．5％），并显著优于对照药物甘乐组。结论：祛湿化瘀方对单纯高脂饮食诱导的非酒精性脂肪肝有理想疗效，值得深入研究。

Effect of Qushi Huayu decoction on prevention and treatment of fatty liver induced by high-fat diet in rats

Objective:To study the experimental efficacy of Qushi Huayu decoction on prevention and treatment of non-alcoholic fatty liver(NAFL).Methods:Thirty six male SD rats,5 rats among them were taken randomly as normal control group,the others were established models by only giving high-fat diet which contained 88% common feedingstuff,2% cholesterin and 10% lard for 12 weeks.From the seventh week all rats established models were divided into model group(n=11),Qushi Huayu Decoction group(n=10) and Ganle treated comparison group(n=10) and were given corresponding medicine or distilled water via gastrogavage.After the rats were sacrificed and materials were collected,the followings were detected: liver pathology,the ultrastructure of liver cells,the content of liver and serum triglyceride(TG) and free fatty acid(FFA),the serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST) and HOMA-insulin resistance(HOMA-IR).Results: The livers of the model group were fatter and bigger than those of the normal group.There were significant hepatic steatosis in the model group;the ultrastructure of liver indicated that there were many fatty drops accumulating together in the plasm of model hepatocyts.Liver content of TG and FFA of the normal group were significantly increased compared with the normal control group(the content of TG and FFA is 5.4 times and 1.7 times as that of the normal control group respectively).Compared with the normal control group,serum ALT,AST activity,and HOMA-IR of the model group had no significant change in this model.Qushi Huayu decoction could improve the liver pathology and significantly decreased the content of liver TG and FFA(the content of TG and FFA of Qushi Huayu decoction group is 54.5% and 72.5% as that of the model group respectively).The treatment efficacy of Qushi Huayu decoction was better than that of Ganle.Conclusion:Qushi Huayu decoction has good experimental efficacy on treatment of NAFL.It is worth further study.