针刺对去势雌性大鼠血清骨钙素的影响

目的　探讨针刺治疗绝经后骨质疏松症的作用机理。方法　采用 6月龄 Wistar雌性大鼠 80只 ,随机分成去卵巢组和假手术组 ,术后 3个月随机抽取 3只去卵巢组大鼠处死后取胫骨上端做骨组织形态学计量检测。然后将去卵巢组大鼠随机分成针刺组和对照组 ,针刺组每日行针刺治疗 1次 ,10次为 1疗程 ,疗程间休息 3d,共治疗 3个疗程 ;对照组不给予任何治疗。最后所有大鼠于股动脉处取血 ,用放免法检测血清骨钙素 ;取胫骨上端做骨组织形态学计量。结果　去卵巢组血清骨钙素显著低于假手术组 (P<0 .0 5 ) ;治疗后针刺组血清骨钙素显著高于对照组 (P<0 .0 5 ) ,与假手术组对比无明显差异。针刺组骨小梁平均宽度、骨小梁面积百分比显著高于对照组 (P<0 .0 5 ) ,与假手术组对比无明显差异 ;针刺组骨髓腔平均横径、骨髓腔面积百分比显著低于对照组 (P<0 .0 5 ) ,与假手术组对比无明显差异。结论　提高血清骨钙素水平是针刺疗法治疗绝经后骨质疏松症的可能机制之一。

The Effect of Acupuncture on Serum Osteo-calcium in Castrated Female Rats

Objective To investigate the mechanism of the curative effect of acupuncture on postmenopausal osteoporosis.Method Eighty 6-month-old Wistar female rats were randomly divided into an ovariectomy group and a sham operation group. Three rats selected from the ovariectomy group 3 months after the operation were sacrificed and the upper part of tibia was taken for an osteo-histomorphological quantitative test. The rats of the ovariectomy group were than randomly divided into an acupuncture group and a control group. The acupuncture group was needed once daily, 10 times as a course, for a total of 3 courses. There was a 3-day interval of rest between the courses of treatment. The control group was not given any treatment. Finally, blood was taken from the rat's femoral artery and serum osteo-calcium measured by radioimmunoassay, and the upper part of tibia was taken for osteo-histomorphological measurement.Results Serum osteo-calcium was significantly lower in the ovariectomy group than in the sham operation group (P<0.05). After the treatment, serum osteo-calcium in the acupuncture group was significantly higher than that in the control group (P<0.05) and not significantly different from that in the sham operation group. The average width and the area percentage of bone trabecula in the acupuncture group were significantly larger than those in the control group (P<0.05) and not significantly different from those in the sham operation group. The average diameter and the area percentage of the marrow cavity in the acupuncture group were significantly larger than those in the control group (P<0.05) and not significantly different from those in the sham operation group.Conclusion Raising serum osteo-calcium level may be one of the mechanisms by which acupuncture treats postmenopausal osteoporosis.