用荧光原位杂交技术检测乳腺癌细胞系第6号染色体长臂缺失

目的 研究１０例乳腺癌细胞系的６号染色体第臂缺失。方法 运用荧光原位杂交（ｆｌｕｏｒｅｓｃｅｎｃｅ ｉｎ ｓｉｔｕ ｈｙｂｒｉｄｉｚａｔｉｏｎ，ＦＩＳＨ）技术，并采用３７个分布于６ｑ１２－６ｑ２７的ＹＡＣ探针和６号染色体着丝粒特异性探针，检测了１０例乳腺癌细胞系的６号染色体长臂。结果 在５例细胞系中发现６ｑ１２－６ｑ２７的大段缺失，１例发现有６ｑ２５－ｑ２７的小段缺失。在含有多个细胞亚群的两侧细胞

Delineation of 6q deletions in breast carcinoma cell lines by fluorescence in situ hybridization

Objective To detect and delineate 6q deletions in ten breast carcinoma cell lines. Methods Fluorescence in situ hybridization (FISH) with 37 YAC probes hybridizing from 6q12 to 6q27 and a centromeric probe of chromosome 6 as internal control.Results In five cell lines, large deletions extending from 6q12 to 6q27 were found, while a small deletion involving 6q25 6q27 was identified in one cell line. In two cell lines containing different cell populations, deletions of 6q occurred in cells with polysomy 6, but not in cells with disomy 6. In addition, an overpresentation of 6q21 q22.2 was detected in one cell line. Conclusion 6q25 q27 seems to be the commonly deleted region in breast cancers and 6q deletions might be secondary changes.