支气管哮喘患儿与RANTES单核苷酸多态性的相关性研究

目的探讨RANTES单核苷酸多态性(SNP)与哮喘易感患儿的相关性.方法采用聚合酶链反应方法对32例哮喘患儿和32例正常对照进行了RANTES-403及-28位点基因型分布和等位基因频率研究.结果病例组RANNTES-403位点G/G、G/A、A/A基因型频率分别为53.13%、40.62%、6.25%;对照组分别为50.00%、43.75%、6.25%(P＞0.05).两组突变等位基因-403A频率分别为26.53%和28.13%(P＞0.05).病例组RANTES-28位点C/C、C/G、G/G基因型频率分别为78.13%、18.75%、3.12%;对照组分别为90.63%、9.37%、0%(P＞0.05).两组突变等位基因-28G频率分别为12.50%和4.69%(P＞0.05).结论RANTES-403位点和-28位点SNP与哮喘发病可能没有直接的关系.

The SNPs of chemokine RANTES promoter in children with asthma

Objective:To detect the association between the single nucleotide polymorphism (SNPs)of chemokine RANTES in children with asthma. Methods:We use PCR- RFLP to study the SNPs of RANTES polymorphism at - 403 and - 28 sites in the blood specimens of 32 children with asthma and 32 controls. Results:The genotyping frequencies of RANTES- 403 G/G,G/A and A/A in case group were 53.13% , 40.62% and 6.25%, respectively; while the genotyping frequencies of RANTES- 403 G/G.G/A and A/A in control were 50.00% ,43.75% and 6.25%, respectively( P>0.05) .There is no significantly difference in the frequency of mutation - 403A allele between case group and controls (26.53% vs 28.13%, P>0.05). The genotyping frequencies of RANTES - 28 C/C,C/G and G/G in case group were 78.13%, 18.75 % and 3.12%, respectively; which were not significantly different from those in controls as 90.63% ,9.37% and 0% (P>0.05). There is no significantly difference in the frequency of mutation - 28G allele between asthma and controls (12.50% vs 4.69% , P>0.05). Conclusions:These results indicate that the SNPs of RANTES promoter are may not associated asthma.