坚龙胆主要次生代谢产物及分子差异的研究

目的:通过含量测定与DNA条形码分析,探讨坚龙胆主要次生代谢产物及分子的差异。方法:采用HPLC测定不同居群坚龙胆根及根茎、茎、叶、花中主要次生代谢产物龙胆苦苷、獐芽菜苦苷、獐芽菜苷及苦龙胆酯苷含量,建立其指纹图谱共有模式,用单因素方差分析对主要次生代谢产物间的差异进行评价;并采用DNA条形码技术对不同居群坚龙胆样品进行ITS2测序。结果:不同居群坚龙胆根及根茎、茎、叶、花中龙胆苦苷、獐芽菜苦苷、獐芽菜苷及苦龙胆酯苷含量具有极显著差异(P0.01);同时ITS2序列结果显示不同居群坚龙胆分子差异较小。结论:不同居群坚龙胆主要次生代谢产物有一定差异,但分子间差异较小,亲缘关系较近。

Research on Differences Between Main Secondary Metabolites and Molecules of Gentiana rigescens

Objective: To investigate the differences between main secondary metabolites and molecules of Gentiana rigescens from different populations by the method of content determination and DNA barcoding analysis. Method: An HPLC method was applied to determine the contents of gentiopicroside, swertiamarin, sweroside and amarogentin in G. rigescens roots and rhizomes, stems, leases, and flowers, and the HPLC fingerprint was established, the main secondary metabolites were evaluated by one-way ANOVA, and the DNA barcoding technique was used to test ITS2 sequences of G. rigescens. Result: There were significant differencedifferences in the contents of gentiopicroside, swertiamarin, sweroside and amarogentin (P<0.01) in different parts.fractions. The results of ITS2 sequences showed that there was no significant difference in the molecule of G. rigescens from different populations. Conclusion: There was differences in main secondary metabolites of G. rigescens from different populations, but there was little difference of with less differences among molecules and a close genetic relationship.