藜蒿叶水提物对白色念珠菌的抗菌效应及强化措施

目的:研究醇沉处理和中药配伍对藜蒿叶水提物抑制白色念珠菌生长作用的影响。方法:采用微量液基稀释法测定不同醇沉浓度纯化处理后的藜蒿叶水提物对白色念珠菌的抑制作用,包括对其菌体生长量、生物膜形成、芽管生成作用等方面的影响,并考察中药配伍对藜蒿叶水提物抗白色念珠菌活性的影响。结果:醇沉处理可显著降低藜蒿叶水提物对白色念珠菌的最小抑菌浓度(MIC),醇沉浓度90%处理使MIC80(下降80%的药物质量浓度)由200 g·L~(-1)降至8 g·L~(-1),同时增强了其对白色念珠菌芽管生成和生物膜形成作用的抑制效果;在选取的18种常见植物药材中,薰衣草、黄芩、莪术对藜蒿叶水提物表现出了显著的抑菌增效作用(部分抑制浓度指数0.5),与黄芩联用时藜蒿叶水提物的MIC80最小,达0.60 g·L~(-1)。结论:醇沉处理和中药配伍可显著提高藜蒿叶水提物对白色念珠菌的抑菌效果,为藜蒿副产物资源的综合利用提供参考。

Antifungal Activity and Strengthening Measures of Water Extract of Artemisia selengensis Leaves on Candida albicans

Objective: To investigate the effect of alcohol precipitation and compatibility on inhibition of water extract of Artemisia selengensis leaves for Candida albicans. Method: Inhibition of water extract after alcohol precipitation with different concentration for C.albicans was determined by broth microdilution assay,including the inhibition of biomass growth,biofilm formation and germ tube formation.And the effect of compatibility between water extract and other herbs on activity of C.albicans was investigated. Result: Alcohol precipitation significantly reduced the minimum inhibitory concentration (MIC) of water extract to C. albicans,alcohol precipitation with 90%can let MIC₈₀ (down 80%of drug concentration) down from 200 g·L^-1 to 8 g·L^-1.Besides,the inhibition of biofilm formation and germ tube formation of C. albicans were improved.Among these selected 18 kinds of herbs,Lavandula angustifolia,Scutellaria baicalensis and Curcuma phaeocaulis showed significant synergistic effect for inhibition of C. albicans(factional inhibitory concentration index was less than 0.5).MIC₈₀ of water extract was reduced to 0.60 g·L^-1 when combined with S. baicalensis. Conclusion: Alcohol precipitation and compatibility of water extract of A. selengensis leaves can improve its inhibitory activity for C. albicans.