| 中国人MBL cDNA的克隆与序列分析 |
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| 引用本文： | 陈政良,朱锡华,谢佩蓉. 中国人MBL cDNA的克隆与序列分析[J]. 免疫学杂志, 1999, 15(2): 83-86 |
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| 作者姓名： | 陈政良 朱锡华 谢佩蓉 |
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| 作者单位： | 1. 第一军医大学免疫学教研室
2. 第三军医大学免疫学教研室,重庆,400038 |
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| 基金项目： | 全军重点实验室项目基金 |
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| 摘 要： | 从中国汉族人胎肝组织提取ＲＮＡ，以ＲＴ－ＰＣＲ方法获得了编码含号顺序的全长甘露聚糖结合凝集素肽链的ｃＤＮＡ片段，将其与ｐＧＥＭ－Ｔ载体连接，转化大肠杆菌ＴＧ１，
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| 关 键 词： | 甘露聚糖结合凝集素 cDNA克隆 序列分析 |
CLONING AND SEQUENCING OF MANNAN-BINDING LECTIN cDNA OF CHINESE |
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| Chen Zhengliang,Zhu Xihua,Xie Peirong. CLONING AND SEQUENCING OF MANNAN-BINDING LECTIN cDNA OF CHINESE[J]. Immunological Journal, 1999, 15(2): 83-86 |
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| Authors: | Chen Zhengliang Zhu Xihua Xie Peirong |
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| Abstract: | The intact cDNA encoding the mannan binding lectin polypeptide including the signal sequence was isolated by using RT PCR method with total RNA extracted from fresh liver tissue of a Han nationality foetus, linked into pGEM T vector and transformed into E.coli TG1. The result of sequencing indicated that the sequence of our cDNA is different greatly from that of the reported human MBL cDNA but identical with that of the mRNA for human MBL in GenBank except two nuc leotides, and that its encoded product consists of a typical hydrophobic signal sequence of 20 amino acids and a mature MBL polypeptide of 228 amino acids. |
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| Keywords: | RT-PCR |
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