| 桂枝配方颗粒的HPLC指纹图谱研究 |
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| 引用本文： | 江洁怡,;董玉娟,;李养学,;李素梅,;胥爱丽. 桂枝配方颗粒的HPLC指纹图谱研究[J]. 长春中医药大学学报, 2014, 0(6): 1015-1018 |
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| 作者姓名： | 江洁怡, 董玉娟, 李养学, 李素梅, 胥爱丽 |
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| 作者单位： | [1]广东省中医药工程技术研究院,广州510095; [2]广州中医药大学,广州510405 |
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| 基金项目： | 广东省教育部科技部产学研结合项目（2011A091000005）; 广东省科技厅中药配方颗粒生产和质量标准化关键技术研究167（2012A030100017） |
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| 摘 要： | 目的建立桂枝配方颗粒的高效液相指纹图谱,为桂枝配方颗粒的鉴别及其质量控制提供实验依据。方法采用高效液相色谱（HPLC）法,Agilent Zorbax SB-C18（4.6 mm×250 mm,5μm）为色谱柱;乙腈-0.1%磷酸溶液为流动相进行梯度洗脱,检测波长为280 nm;柱温20℃;流速0.8 m L/min。结果建立了桂枝配方颗粒的高效液相指纹图谱,以16号峰为参照峰确定了桂枝配方颗粒的16个共有峰,并指认出了原儿茶酸、肉桂酸,对照指纹图谱色谱峰丰富,分离度高,相似性好。结论该方法方便可靠,可用于桂枝配方颗粒的鉴别、质量评价与控制。
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| 关 键 词： | 桂枝配方颗粒 HPLC 指纹图谱 原儿茶酸 肉桂酸 |
HPLC fingerprint analysis of cinnamomi ramulus formula granules |
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| Affiliation: | JIANG Jieyi, DONG Yujuan, LI Yangxue, LI Sumei, XU Aili ( 1. Guangdong Province Engineering Technology Research Institute of T. C. M, Guangzhou 510095, China; 2. Guangzhou University of Chinese Medicine, Guangzhou 510405, China) |
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| Abstract: | Objective To establish the fingerprint of Cinnamomi Ramulus Formula Granules for identifying and evaluating its quality by HPLC. Methods The HPLC analysis was carried out in Agilent Zorbax SB-C18（4.6 mm × 250 mm, 5 μm） with a mobile phase of acetonitrile - 0.1% phosphoric by gradient elution. The detection wavelength was 280 nm; the column temperature was 20 ℃ and the flow rate was 0.8 mL· min^-1. Re,tilts 16 peaks were selected as the character fingerprint peaks of Cinnamomi Ramulus Formula Granules with the identified of Protocatechuic acid and Cinnamic acid. Good similarities were found in the established fingerprint through similarity analysis and the resolution was good. Conclusion The method is convenient and reliable and can be used as the quality control for Cinnamomi Ramulus Formula Granules. |
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| Keywords: | cinnamomi ramulus formula granules HPLC fingerprints protocatechuic acid cinnamic acid |
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