转染人白介素-10基因对未成熟心肌缺血再灌注损伤的影响

目的:观察转染人白细胞介素10(hIL-10)基因对未成熟心肌缺血再灌注(IR)的影响并探讨其可能作用机制。方法:24只3~4周龄的幼兔被随机分为空白对照组、空载对照组和基因转染组,每组8只。以阻断左冠左室支30 min再开放120 min,建立IR动物模型;阻断前经左室内注射重组腺病毒转染hIL-10。结果:IR后,空载对照组的血流动力学各项指标恢复率明显差于空白对照组(P均0.05),基因转染组的各项指标明显优于空载对照组(P0.05);与空白对照组比较,空载对照组和基因转染组的丙二醛(MDA)含量[(6.33±1.03)μmol/gwet∶(14.35±1.28)μmol/g wet∶(8.78±1.22)μmol/g wet]和髓过氧化物酶(MPO)活性[(5.42±0.72)U/g wet∶(19.35±2.32)U/g wet∶(9.26±1.26)U/g wet]明显升高(P均0.05),超氧化物歧化酶(SOD)活性明显下降[(215.25±26.65)U/g wet∶(93.82±12.61)U/g wet∶(176.33±19.84)U/g wet,P均0.05];与空载对照组比较,基因转染组的MDA含量和MPO活性明显下降,SOD活性明显升高(P均0.05)。结论:经左室内注射重组腺病毒载体介导hIL-10转基因有效可行,可减轻未成熟心肌缺血再灌注损伤。

Effect of gene transfer of human interleukin-10 on ischemia reperfusion injury in immature myocardium

Objective： To investigate the effect of adenovirus-mediated gene transfer of human interleukin-10 on immature myoeardium after regional myocardial ischemia-reperfusion, and discuss the mechanisms. Methods： Twenty-four immature rabbits were randomly divided into three groups （8 animals/group） ： group A （control group）, group B （no receiving adenovec-hIL-10 complex）, group C （adenovirus-mediated gene transfer of human interleukin-10 was injected into left ventricles before ischemia）. Left coronary artery was temporarily occluded for 30 min, followed by 120 min reperfusion for astabish IR mode. The transgene expression of hIL-10 was detected by means of immunohisto chemistry. Result： After IR, the indexes of hemodynamies of group B were significantly worse than those of group A （P〈0.05 all）, those of group C were better than those of group B （P〈0.05）. Compared with group A, the level of malonyl diadehyde （MDA） [ （6.3±31.03） μmol/g wet vs. （14.35±1.28） μmol/g wet vs. （8.78±1.22） μmol/g wet], myeloperoxidase （MPO） [ （5.42±0.72） U/g wet vs. （19.35±2.32） U/g wet vs. （9.26±1.26） U/g wet ] significantly increased , s uperoxide dismutase （SOD） [ （ 215.25 ± 26.65） U/g wet vs. （ 93.82 ±12.61） U/g wet vs. （176.33± 19. 84） U/g wet significantly decreased ], P〈0.05 all in group B, group C. Compared with group B, the content of MDA, and activity of MPO significantly decreased, the SOD activity significantly increased in group C, P〈 0.05 all. Conclusions： Adenovirus-mediated gene transfer of human interleukin-10 in immature myocardium may ameliorates regional ischemia-reperfusion injury.