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| 短发夹RNA靶向沉默TPX2基因促进人肺腺癌A549细胞凋亡及其相关机制 | |
| 引用本文： | 刘莹,张徽,唐熹. 短发夹RNA靶向沉默TPX2基因促进人肺腺癌A549细胞凋亡及其相关机制[J]. 肿瘤, 2011, 31(12): 1055-1060. DOI: 10.3781/j.issn.1000-7431.2011.12.001 |
| 作者姓名： | 刘莹 张徽 唐熹 |
| 作者单位： | 重庆医科大学分子医学与肿瘤研究中心病理学教研室,重庆,400016 |
| 摘 要： | 目的:探讨靶向Xklp2靶蛋白(targeting protein for Xenopus kinesin-like protein2,TPX2)基因的短发夹RNA(short hairpin RNA,shRNA)对肺腺癌A549细胞凋亡的影响及其可能机制。方法:构建靶向TPX2基因的shRNA重组载体,将其转染至肺腺癌A549细胞中,RT-PCR检测细胞中TPX2、Aurora-A、p53和Bcl-2mRNA的表达,蛋白质印迹法检测TPX2蛋白的表达,FCM检测细胞周期和细胞凋亡情况。结果:成功构建重组载体pMagic4.1-shRNA-TPX2。将pMagic4.1-shRNA-TPX2转染至A549细胞后,TPX2、Aurora-A和Bcl-2mRNA的表达水平明显下调,p53mRNA的表达水平明显上调,TPX2蛋白的表达水平明显下调,细胞凋亡率明显增加,细胞阻滞于S期,与空白对照组(未转染组)和阴性对照组(转染pMagic4.1-shRNA-NC)相比,差异均有统计学意义(P<0.05)。结论:靶向TPX2的shRNA能促进肺腺癌A549细胞的凋亡,其作用可能与上调p53表达和下调Bcl-2表达有关。 |
| 关 键 词： | 肺肿瘤 RNA干扰 细胞凋亡 基因,TPX2 |
TPX2 gene-targeted short hairpin RNA induces the apoptosis of human lung adenocarcinoma A549 cells and its related mechanism |
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| LIU Ying,ZHANG Hui,TANG Xi. TPX2 gene-targeted short hairpin RNA induces the apoptosis of human lung adenocarcinoma A549 cells and its related mechanism[J]. Tumor, 2011, 31(12): 1055-1060. DOI: 10.3781/j.issn.1000-7431.2011.12.001 | |
| Authors: | LIU Ying ZHANG Hui TANG Xi |
| Affiliation: | LIU Ying,ZHANG Hui,TANG Xi Department of Pathology,Research Centre of Molecular Medicine and Cancer,Chongqing Medical University,Chongqing 400016,China |
| Abstract: | Objective:To investigate the effect of short hairpin RNA(shRNA) targeting Xenopus kinesin-like protein 2(TPX2) gene on the apoptosis of human lung adenocarcinoma A549 cells and to explore its possible mechanism.Methods:The combination vector of shRNA targeting TPX2 gene was established and then it was transfected into A549 cells.The expression levels of TPX2,Aurora-A,p53 and Bcl-2 mRNAs in A549 cells after transfection with combination vector were detected by RT-PCR,and the expression level of TPX2 protein ... |
| Keywords: | Lung neoplasms RNA interference Apoptosis Gene TPX2 |
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