消癌平联合奥曲肽对H22肝癌荷瘤小鼠Th1/Th2类细胞因子的影响

目的:观察消癌平注射液(Xiaoaiping injection,XAP)联合奥曲肽(octreotide,OCT)对H22肝癌的抑瘤作用,寻找两药联合作用的最佳药效浓度,并探讨其可能的作用机制。方法:建立小鼠皮下H22移植癌模型。成瘤后将实验动物随机分为正常组,模型组,XAP低、中、高剂量组,OCT组,XAP低、中、高剂量分别联合OCT组。各组药物处理后,测量小鼠皮下肿瘤大小,绘制肿瘤的生长曲线。剥瘤后称取瘤体质量,计算小鼠的肿瘤抑制率;行肿瘤组织病理形态学及HE染色观察;并用MTT法检测各组脾脏T淋巴细胞的增殖能力;ELISA法检测1包括白细胞介素2(interleukin-2,IL-2)和干扰素γ(interferon-γ,IFN-γ)]和2(包括IL-4和IL-10)两类细胞因子的产生水平。结果:各组治疗药物对H22肝癌的生长均有一定的抑制作用,联合用药组的抑瘤作用优于单药组(P<0.05),可见XAP+OCT联用具有协同作用,其中以高剂量XAP+OCT的抑瘤作用最好。模型组小鼠的T细胞免疫功能较正常对照组明显下降(P<0.05),并表现为IFN-γ、IL-2分泌量下降,IL-4、IL-10含量升高,呈现2型应答状态。药物干预后各组T细胞增殖能力均有一定程度的增强,以联合用药组增强最为明显,而且联合用药组纠正细胞因子的失衡作用明显优于单独用药组。结论:高剂量XAP联合OCT抑瘤效果最佳,其抑制肿瘤生长的作用可能与增强H22荷瘤T细胞的活性、有效纠正荷瘤导致的1/2失衡以及增强机体的抗肿瘤免疫功能等有关。

The inhibitory effect of Xiaoaiping injection combined with octreotide on the expression of cytokines Th1/Th2 in H22 liver cancer in mice

Objective:To observe the inhibitory effect of Xiaoaiping injection(XAP) combined with octreotide(OCT) on the growth of H22 liver cancer in mice and determine the best dose for combined medication,then to explore its possible mechanism.Methods:A H22 subcutaneous tumor model was established in Kunming mice.After tumor formation,the mice were randomly divided into untreated group,XAP-treated group with low-,medium-or high-dose,OCT-treated group,and the combined medication group with low-,medium-or high-dose of...