| 卵巢癌组织及腹腔积液中肿瘤干细胞的分离与培养 |
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| 引用本文： | 施敏凤,谢幸,朱曼,吕卫国,叶枫,焦洁.卵巢癌组织及腹腔积液中肿瘤干细胞的分离与培养[J].肿瘤,2011,31(10):906-910. |
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| 作者姓名： | 施敏凤 谢幸 朱曼 吕卫国 叶枫 焦洁 |
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| 作者单位： | 1. 第二军医大学附属长海医院妇产科,上海,200433
2. 浙江大学医学院附属妇产科医院妇瘤科,杭州,310006 |
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| 摘 要： | 目的:从人卵巢癌组织和腹腔积液中分离培养肿瘤干细胞,分析细胞表型和化疗药物敏感性。方法:取卵巢浆腺癌患者的原发肿瘤组织及腹腔积液,通过无血清培养形成悬浮生长的球体。采用FCM及免疫荧光法检测干细胞标志物的表达。体外药敏实验检测获得的球体细胞和分化细胞对紫杉醇和卡铂的药物敏感性。结果:卵巢癌组织及腹腔积液中的卵巢癌细胞经无血清悬浮培养可以形成球体,后者具有CD44+CD24-表型并表达Oct4、Nanog及Sox2等干细胞标志物;腹腔积液及组织来源的球体细胞经血清培养液培养3周后发生分化,CD44+CD24-细胞比例分别下降至47%和3%;分化细胞对紫杉醇和卡铂的敏感性均显著高于球体细胞(P<0.01)。结论:卵巢癌的肿瘤干细胞属于CD44+CD24-细胞,后者表达Oct4等干细胞标志并具有显著耐药性。
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| 关 键 词： | 卵巢肿瘤 干细胞 球体 药物筛选试验 抗肿瘤 |
Isolation and culture of cancer stem cells from ovarian cancer tissues and malignant ascites |
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| SHI Min-feng,XIE Xing,ZHU Min,L Wei-guo,YE Feng,JIAO Jie.Isolation and culture of cancer stem cells from ovarian cancer tissues and malignant ascites[J].Tumor,2011,31(10):906-910. |
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| Authors: | SHI Min-feng XIE Xing ZHU Min L Wei-guo YE Feng JIAO Jie |
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| Institution: | SHI Min-feng,XIE Xing,ZHU Min,L(U) Wei-guo,YE Feng,JIAO Jie |
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| Abstract: | Objective:To isolate and culture tumor stem cells from ovarian cancer tissues and malignant ascites,and to analyze the cellular phenotype and drug sensitivity.Methods:The primary tumor tissues and the malignant ascites samples from one patient with ovarian adenocarcinoma were collected,and the formations of float-growing spheres was developed with serum-free medium culture.The expressions of stem cell markers were evaluated by flow cytometry(FCM) and immunofluorescent staining.The sensibilities of sphere ce... |
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| Keywords: | Ovarian neoplasms Stem cells Sphere Drug screening assays antitumor |
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